Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 2R44AR044601-02A2
Agency Tracking Number: AR044601
Amount: $830,929.00
Phase: Phase II
Program: SBIR
Awards Year: 2002
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 (508) 881-6771
Business Contact
Phone: (508) 881-6771
Research Institution
DESCRIPTION (provided by applicant): Phase I research demonstrated that a Langerhans cell (LC) containing model of the epidermis is feasible. LC progenitor cells were harvested from peripheral or cord blood samples, cryopreserved, and matured using cytokines into the LC specific CD1a+ phenotype. By modifying the extracellular matrix substrate, these cells were successfully incorporated into a highly differentiated, serum-free epidermal model, albeit at a lower density than in native epidermis. The LC derived from cord blood maintained their dendntic, CD1a+ HLA-DR+/- phenotype and expressed Birbeck granules within the cultures. The exposure of 2 contact allergens resulted in the release of TNF-a and IL-lb. 2 cytokines known to be critically involved in the initial stages of allergic contact dermatitis (ACD). Exposure to the common irritant, on the other hand, did not result in TNF-a or IL- lb release. In addition, following exposure to UV irradiation, immuno-staining showed that LC were migrating out of the tissue, as is known to occur in vivo.Phase II studies will expand on the Phase I results to further develop an epidermal model containing immunologically competent cells. Initial research will focus on the optimization of culture conditions to improve LC incorporation into the tissue model. The effects of allergens on cytokine release and gene expression will be investigated. Experiments designed to model LC migration from skin following exposure to allergens or UV light and subsequent T-cell activation will be performed to determine the degree to which the model's behavior corresponds to in vivo ACD reactions. Finally, based on these studies, an assay kit to predict whether a material is likely to act as a contact allergen will be developed.

* Information listed above is at the time of submission. *

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government