IN VITRO SKIN MODEL FOR PERCUTANEOUS ABSORPTION STUDIES

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43AR048007-01A1
Agency Tracking Number: AR048007
Amount: $181,352.00
Phase: Phase I
Program: SBIR
Awards Year: 2002
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
MATTEK CORPORATION
MATTEK CORPORATION, 200 HOMER AVE, ASHLAND, MA, 01721
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 MITCHELL KLAUSNER
 (508) 881-6771
 MKLAUSNER@MATTEK.COM
Business Contact
 JOHN SHEASGREEN
Phone: (508) 881-6771
Email: JSHEASGREEN@MATTEK.COM
Research Institution
N/A
Abstract
DESCRIPTION (provided by the applicant): The ability to produce skin in vitro with barrier properties equivalent to those of native epidermis remains a much sought after goal. The current proposal will utilize a number of recent improvements in the organotypic culture of skin cells to optimize the barrier properties of a highly differentiated, serum-free skin model. Medium additives, methods of culture, and the original cell source from which the epidermal cells were derived will be investigated in order to reconstruct in vivo-like skin. The modified skin tissues will be characterized in terms of histology, surface hydration, lipid content, and ultrastructure. Finally, the permeability characteristics of the in vitro tissues in relation to three model drugs spanning a range of lipophilicity will be investigated. PROPOSED COMMERCIAL APPLICATION: The improved skin tissue resulting from this research will find utilization in transdermal drug delivery studies, risk assessment related to accidental or occupational exposure of chemicals to the skin, and skin irritation studies. In addition, the methodology developed will likely improve cultured skin graft material since it has ben reported that skin tissue with improved barrier properties increases the efficacy of such grafts.

* information listed above is at the time of submission.

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