ENZYME TAGGING OF MALIGNANT CERVICAL EPITHELIAL CELLS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$49,660.00
Award Year:
1992
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
19067
Agency Tracking Number:
19067
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
3139 South Wayne Road, Wayne, MI, 48184
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Daisy S Mccann
(313) 721-0300
Business Contact:
() -
Research Institute:
n/a
Abstract
THE LONG-TERM AIM OF THIS PROJECT IS THE DEVELOPMENT OF AN AUTOMATED SCREENING INSTRUMENT (REAGENTS INTEGRATED WITH INSTRUMENTATION) FOR PAP SMEARS. PHASE I WILL BE USED TO DEMONSTRATE THE FEASIBILITY OF USING 2 MONOCLONAL ANTIBODIES AGAINST 2 ENZYMES AS THE KEY REAGENTS IN THE SYSTEM. BRIEFLY THE PAP SMEAR WILL BE ALLOWED TO INTERACT WITH TWO MONOCLONAL ANTIBODIES EACH CARRYING A DISTINCTIVE FLOURESCENT REPORTER MOLECULAR. THESE ANTIBODIES HAVE BEEN SELECTED TO IDENTIFY AND QUANTITATE LACTATED DEHYDROGENASE (LD) AND ADENYL CYCLASE (AC) RESPECTIVELY. LD OCCURS IN HIGHER THAN NORMAL, AC IN LOW TO NORMAL CONCENTRATIONS IN NEOPLASTIC CELLS. A FLUOROMETER CAPABLE OF READING TWO DISCREET WAVELENGTHS SIMULTANEOUSLY, CAN THEREFORE DEVELOP A NUMERICAL RATIO, WHICH WILL ASSESS THE DEGREE TO WHICH NEOPLASIA IS REPRESENTED IN A GIVEN SLIDE. BASED ON THESE RATIOS, NORMAL SLIDES (85-90% OF ALL THE SLIDES) WILL NEED NO FURTHER EVALUATION, ABNORMAL SLIDES ONLY, WILL NEED TO BE READ BY THE CYTOTECH/CYTOLOGIST.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government