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Device for the Uncontaminated Collection of Multiple Microbial Samples

Award Information
Agency: Department of Commerce
Branch: N/A
Contract: N/A
Agency Tracking Number: 43563
Amount: $59,908.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Solicitation Year: N/A
Award Year: 1998
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
121 Bernard E. St. JEAN DRIVE
East Falmouth, MA 02536
United States
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
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Business Contact
Phone: () -
Research Institution

The objective of this research project is the development of an Autonomous Microbial Sampler (AMS) that
will obtain uncontaminated and exogenous DNA-free microbial samples from deep-sea hydrothermal vents
and other aquatic ecosystems. Sampling with the AMS may be conducted using manned submersibles,
Remotely Operated Vehicles (ROV), Autonomous Underwater Vehicles (AUV) or during hydrocast
operations on ships. The modular device consists of a titanium sample nozzle for sampling potentially hot
samples (-250 C) and fluid handling components housed within a stainless steel frame for collection of six
independent filtered or unfiltered samples. An on board microcomputer permits sampling to be controlled by
the investigator, external devices (e.g. AUV computer) or internal programming. Temperature, volume
pumped and other parameters are recorded during sampling. Detailed engineering design of the device and
choice of construction of a prototype AMS, and a program of thorough laboratory and field testing of it¿s
operation. Minor design changes based on testing results will be incorporated into the device. Field testing
will be conducted during an upcoming R/V Atlantis/DSV Alvin cruise to a deep water hydrothermal vent site
off the U.S. or Mexican West coast in spring 2000. Test hydrocast water column sampling will be
conducted during an R/V Endeavor cruise to the Sargasso Sea in spring 2001.

* Information listed above is at the time of submission. *

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