Rapid homogeneous antibody-based detection of proteins

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$954,812.00
Award Year:
2009
Program:
STTR
Phase:
Phase II
Contract:
2R42GM079891-02
Award Id:
85692
Agency Tracking Number:
GM079891
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
815 WENNEKER DRIVE, SAINT LOUIS, MO, 63124
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
100512883
Principal Investigator:
TOMASZ HEYDUK
(314) 977-9238
HEYDUKT@SLU.EDU
Business Contact:
YIE CHANG
() -
yiechang@mediomics.com
Research Institute:
SAINT LOUIS UNIVERSITY

221 N GRAND BLVD
SAINT LOUIS, MO, 63103 3098

Nonprofit college or university
Abstract
DESCRIPTION (provided by applicant): Detection and quantification of proteins in various biological samples is one of the most important and most often utilized assays with applications in research, medical diagnosis, early disease detection and detection of biological threat agents. While many protein detection methodologies are available (ELISA being a gold standard of those), they each have some limitations. The most common limitation is the time required to perform the assay and the complexity of the sa mple manipulation involved in the assay. Thus, there is a clear need for developing new rapid and homogenous protein detection methodologies. In Phase I of this project we established feasibility of a novel antibody-based homogenous protein detection metho dology (molecular pincers) which allows detection of the presence of a target protein by a simple fluorescence intensity measurement. The assay is rapid and is technically extremely simple to perform (involves essentially no sample manipulation other then mixing the sample with the detection reagent). These characteristics make this new assay format particularly useful in situations where quick determination of the target protein is essential without a support of a laboratory equipped with sophisticated ins trumentation (as in the case, for example, of point-of-care diagnostic assay). The long-term goal of this project will be to utilize molecular pincers to develop simple, rapid and inexpensive point-of-care diagnostic tests for a panel of cardiac disease ma rkers. The goal of this Phase II project will be to develop molecular pincer assays for six markers of cardiac disease (troponin I, B-type natriuretic peptide (BNP), myoglobin, creatine kinase (CK-MB), C-reactive protein (CRP), and D-dimer) for research ma rket. Completion of this goal will allow initiation of the process of approval of these assays for clinical diagnosis. Additionally, we will work on enhancing molecular pincer assay by developing strategies to improve assay sensitivity. Completion of this goal will enhance the commercialization prospects of molecular pincers by broadening the range of possible targets for which the assay could be developed. PUBLIC HEALTH RELEVANCE: Molecular pincer assays will find wide applications in research and d iagnosis of human disease. In research, it could become more convenient, easier to use, less expensive replacement for currently used assays for detecting proteins. In diagnosis, it will allow more rapid and more straightforward detection of antigens corre lated with human disease.

* information listed above is at the time of submission.

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