USE OF A CCD CAMERA FOR LOW-COST CONFOCAL MICROSCOPY

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 16624
Amount: $500,000.00
Phase: Phase II
Program: SBIR
Awards Year: 1992
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Meridian Instruments, Inc.
2310 Science Parkway, Okemos, MI, 48864
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Burrill, Peter H
 Principal Investigator
 (517) 349-7200
Business Contact
 1 R43 GM45640-01
Phone: () -
Research Institution
N/A
Abstract
CONFOCAL MICROSCOPY IS ONE OF THE MOST SIGNIFICANT ADVANCES IN OPTICAL MICROSCOPY. ITS ABILITY TO VIRTUALLY ELIMINATE OUT-OF-FOCUS BLURRING PERMITS OPTICAL SECTIONING OF CELLS AND THICK TRANSPARENT SPECIMENS. THIS TECHNIQUE IS RAPIDLY BEING ADOPTED FOR THE THREE- DIMENSIONAL VISUALIZATION OF CELLULAR AND TISSUE ORGANIZATION. THE SPECIFIC AIMS OF THIS PROJECT ARE TO DETERMINE THE FEASIBILITY OF USING A SLIT SCANNING METHOD AND A CCD CAMERA TO PRODUCE OPTICAL SECTIONS OF FLUORESCENTLY STAINED BIOLOGICAL SPECIMENS. THE LONG TERM OBJECTIVE IS TO PROVIDE A RAPID, LOW-COST CONFOCAL IMAGING INSTRUMENT THAT CAN BE ATTACHED TO MOST CONVENTIONAL EPI-ILLUMINATION MICROSCOPES. TO THIS END, AN OPTICAL BREADBOARD WILL BE DEVELOPED THAT INCORPORATES A UNIQUE SCANNING DEVICE THAT SIMULTANEOUSLY SCANS A SPECIMEN AND A CCD CAMERA. THE SIMPLICITY OF THIS DESIGN IS EXPECTED TO REDUCE THE COST OF THE SYSTEM, AND THE USE OF A CCD CAMERA WILL PROVIDE THE FLEXIBILITY FOR CONFOCAL AND CONVENTIONAL VIDEO IMAGING. THE COMBINATION OF THESE INNOVATIONS WILL RESULT IN AN AFFORDABLE CONFOCAL MICROSCOPE THAT CAN BE USED FOR RESEARCH IN CELL BIOLOGY, NEUROBIOLOGY, CANCER, HISTOLOGY, PATHOLOGY AND MOLECULAR GENETICS. IN ADDITION, THE COST OF THIS INSTRUMENT SHOULD MAKE THIS TECHNOLOGY MORE AVAILABLE FOR CLINICAL AND INSTRUCTIONAL APPLICATIONS.

* information listed above is at the time of submission.

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