Validation of New Mass Spectrometric Blood Folate Assay

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$538,447.00
Award Year:
1996
Program:
SBIR
Phase:
Phase II
Contract:
1 R43 DK50519-01,
Award Id:
29403
Agency Tracking Number:
29403
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
4200 E 9th Avenue, B-170, Denver, CO, 80262
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Cheruppolil Santhosh-Kuma
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
We will develop methods to accurately measure molar amounts of total folates and individual folatefrom blood or tissues by gas chromatography/mass spectrometry (GCMS) using stable isotope labeled fostandards, which completely corrects for recovery. During Phase I, the intrinsic properties of a nebased assay for total folates will be tested by (a) assessing the feasibility of assaying large numbcomparing the reproducibility and precision of the new assay to a standard folate assay and (c) estaconditions for sample storage and transport. The total blood folate level from 50 individuals will binto 5 pools for precision studies. Within-run and between-run variability of the new method will bemethod. Optimal conditions of storage will be established. The project uses GC/MS technology and thebecome the "gold standard" in measurement of folates.

* information listed above is at the time of submission.

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