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Amplification of Molecular Signals

Award Information
Agency: Department of Defense
Branch: Office for Chemical and Biological Defense
Contract: DAAD19-03-C-0123
Agency Tracking Number: C031-0164
Amount: $69,883.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Solicitation Year: N/A
Award Year: 2003
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
1001 Menaul NE, Suite A, Albuquerque, NM, 87107
DUNS: 016541877
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Ihab Abdel-Hamid
 Project Director
 (505) 314-8100
Business Contact
 Bobbi Keen
Title: Controller
Phone: (509) 222-2015
Research Institution
The goal of the proposed research is to develop a highly sensitive and specific amplification system that can be coupled to any pathogen detection and quantification unit for sensitive real-time detection of biological warfare agents. The system is builtaround a dual-enzyme substrate-recycling electrochemical amplification system coupled to an immunoassay detection technology that has already been proven at a benchtop scale.The specific aims of this effort are:1) Fabrication and testing of a bi-enzyme electrochemical amplification module in a microfluidic format.2) Optimization of the various parameters affecting the performance of the amplification system.3) Demonstration of real-time detection of a model biological warfare simulant such as Bacillus globigii.There are two long-term goals after successful completion of this project. One is integration of automated sample collection utilizing MesoSystems' core technology in aerosol collection and particle concentration. The second is multiplexing of real-timedetection using a more sophisticated microfluidic cartridge. The incorporation of both of these advances will produce an inexpensive, handheld unit that can sample the air in any environment and perform rapid, sensitive detection of any biologicalparticles. The proposed amplification system will be capable of rapidly quantifying the presence of extremely low concentrations of a biological agent in a short amount of time. This product, the simplest version of a potential family of products, would itself bea highly marketable product. Areas of use include emergency response to a possible biological warfare threat and in microbiology laboratories. The system proposed here would be faster, cheaper, and more portable than most other detection schemes,including PCR.

* Information listed above is at the time of submission. *

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