ASEPTIC PRECISELY CUT MACRO/MICRO SLICES OF LIVE TISSUES

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 16650
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1991
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Metal Samples Company
152 Metal Samples Rd P O Bo, X 8, Munford, AL, 36268
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Johnson, Donald G
 Principal Investigator
 (205) 358-4202
Business Contact
 1 R43 GM47164-01
Phone: () -
Research Institution
N/A
Abstract
THE OBJECTIVES OF THIS PROJECT ARE: 1) TO PERFECT A NEW KIND OF MICROTOME INVENTED BY ONE OF US (C.L. KRUMDIECK) FOR THE PURPOSE OF PREPARING ASEPTIC SLICES OF LIVE TISSUES FOR ORGAN CULTURE, AND 2) TO DEVELOP ANCILLARY INSTRUMENTATION FOR THE RAPID PREPARATION OF SMALL SQUARE MICRO-SLICES OF NEARLY IDENTICAL DIMENSIONS SUITABLE FOR CULTIVATION IN MULTI-WELL PLATES. THE USE OF CULTURED ORGAN EXPLANTS, OBTAINED BY SLICING LIVE TISSUES TO AN OPTIMAL THICKNESS THAT MINIMIZES DIFFUSION ARTIFACTS AND MAXIMIZES THE NUMBER OF UNDAMAGED CELLS IN THE EXPLANT, HAS BEEN LIMITED BY THE UNAVAILABILITY OF A SUITABLE MICROTOME. THE METHOD HAS, HOWEVER, NUMEROUS ACTUAL AND POTENTIAL APPLICATIONS (E. G. IN VITRO TOXICOLOGY, PHARMACOLOGY, BIOCHEMISTRY, NEUROCHEMISTRY, NEUROPHYSIOLOGY AND OTHERS) AND OFFERS A SIMPLE APPROACH TO REDUCE THE NUMBER OF EXPERIMENTAL ANIMALS CURRENTLY USED IN BIOMEDICAL RESEARCH. IN ADDITION, IT OFFERS THE POSSIBILITY OF USING HUMAN TISSUES (NORMAL AND/OR NEOPLASTIC) OBTAINED DURING SURGERY OR EX CADAVER FOR STUDIES THAT AVOID THE NEED TO EXTRAPOLATE FROM ANIMALS TO HUMANS. SPECIFICALLY, WE WILL: 1) FABRICATE AND EVALUATE THE PERFORMANCE OF NEW PROTOTYPES OF THE KRUMDIECK MICROTOME TESTING DIFFERENT MATERIALS AND INCORPORATING DESIGN IMPROVEMENTS; AND 2) FABRICATE AND EVALUATE A TISSUE-DICING INSTRUMENT THAT, USED IN CONJUNCTION WITH THE SLICER, WILL PERMIT THE PREPARATION OF HUNDREDS OF MICRO-SLICES FROM SMALL (APPROXIMATELY 1.0 CM") SAMPLES OF TISSUE.

* information listed above is at the time of submission.

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