LOCI FOR USE IN ROUTINE SCREENING IN THE L5178Y MOUSE LYMPHOOUR AIM IS TO DETERMINE WHETHER MUTATION AT THE TK LOCUS IN L5178Y MOUSE LYMPHOMA CELLS IS REPRESENTATIVE OF MUTATION THROUGHOUT THE ENTIRE MAMMALIAN GENOME.

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$49,311.00
Award Year:
1985
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
3157
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Microbiological Associates
5221 River Rd., Bethesda, MD, 20816
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
ANDREA M. ROGERSBACK
PRINCIPAL INVESTIGATOR
(301) 654-3400
Business Contact:
() -
Research Institution:
n/a
Abstract
LOCI FOR USE IN ROUTINE SCREENING IN THE L5178Y MOUSE LYMPHOOUR AIM IS TO DETERMINE WHETHER MUTATION AT THE TK LOCUS IN L5178Y MOUSE LYMPHOMA CELLS IS REPRESENTATIVE OF MUTATION THROUGHOUT THE ENTIRE MAMMALIAN GENOME. MUTATION INDUCTION WILL BE COMPARED AT THREE LOCI IN L5178Y CELLS AND TWO LOCI IN CHINESE HAMSTER OVARY (CHO) CELLS. CHEMICALS WITH DIFFERENT MODES OF ACTION IN THE CELL WILL BE EXAMINED. IT IS EXPECTED THAT EACH LOCUS WILL HAVE A DIFFERENT RESPONSE PATTERN TO DIFFERENT CHEMICAL CLASSES. THE GOAL OF THE STUDY IS TO PROVIDE EVIDENCE FOR THE USE OF THE L5178Y TK/ MUTAGENESIS ASSAY IN THE DETECTION OF POTENTIAL CARCINOGENS. THE RESULTS OBTAINED IN PHASE I WITH MODEL COMPOUNDS WILL PROVIDE THE BASIS FOR AN EXTENSIVE STUDY IN PHASE II OF THE RESPONSE OF THE LOCI TO MUTAGENS/CARCINOGENS. THE PROJECT WILL ALSO COMPARE RESULTS OF THE L5178Y MUTAGENESIS ASSAY WITH RODENT CARCINOGENICITY RESULTS TO DETERMINE WHETHER A POSITIVE CORRELATION EXISTS BETWEEN THEM. A POTENTIAL COMMERCIAL APPLICATION OF THE PROJECT IS THE VALIDATION OF MULTIPLE LOCI IN L5178Y CELLS FOR ROUTINE MUTAGENICITY TESTING.

* information listed above is at the time of submission.

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