AN IN VITRO ASSAY FOR TOXIC PARTICULATES

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 11225
Amount: $48,132.00
Phase: Phase I
Program: SBIR
Awards Year: 1989
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
9900 Blackwell Rd, Rockville, MD, 20850
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 () -
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE PURPOSE OF THIS RESEARCH IS TO ESTABLISH A RAPID, INEXPENSIVE, IN VITRO SYSTEM FOR STUDYING THE CYTOTOXICITY AND GENOTOXICITY OF PARTICULATES POTENTIALLY HAZARDOUS TO THE RESPIRATORY SYSTEM. SHORT-TERM TESTS CURRENTLY AVAILABLE TO THE RESEARCH COMMUNITY WERE DESIGNED AND OPTIMIZED USING SOLUBLE TEST MATERIALS AND HAVE BEEN ADAPTED TO THE STUDY OF PARTICULATES WITH LIMITED PHAGOCYTICCAPACITY OF THE TARGET CELLS. THE ASSAY SYSTEM TO BE DEVELOPED WILL USE A FUNCTIONAL MOUSE MACROPHAGE CELL LINE. THIS CELL LINE, RAW264.7, IS AVIDLY PHAGOCYTIC, CAN UNDERGO A RESPIRATORY BURST, AND GROWS READILY IN CULTURE. THIS LATTER FEATURE WILL ALLOW BOTH CYTOTOXICITY AND GENOTOXICITY ENDPOINTS TO BE MEASURED. THE PHASE I GOALS ARE TO: (1) ESTABLISH THE OPTIMAL CONDITIONS FOR A CYTOTOXICITY ASSAY, (2) VALIDATE THE CYTOTOXICITY ASSAY WITH KNOWN TOXIC PARTICULATES, (3) ESTABLISH THE ASSAY CONDITIONS FOR AN HGPRT-BASED FORWARD MUTATION ASSAY, AND (4) ASSAY SEVERAL KNOWN CLASSES OF MUTAGENIC PARTICULATES.

* Information listed above is at the time of submission. *

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