AN IN VITRO ASSAY FOR TOXIC PARTICULATES

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$48,132.00
Award Year:
1989
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
11225
Agency Tracking Number:
11225
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
9900 Blackwell Rd, Rockville, MD, 20850
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
() -
Business Contact:
() -
Research Institute:
n/a
Abstract
THE PURPOSE OF THIS RESEARCH IS TO ESTABLISH A RAPID, INEXPENSIVE, IN VITRO SYSTEM FOR STUDYING THE CYTOTOXICITY AND GENOTOXICITY OF PARTICULATES POTENTIALLY HAZARDOUS TO THE RESPIRATORY SYSTEM. SHORT-TERM TESTS CURRENTLY AVAILABLE TO THE RESEARCH COMMUNITY WERE DESIGNED AND OPTIMIZED USING SOLUBLE TEST MATERIALS AND HAVE BEEN ADAPTED TO THE STUDY OF PARTICULATES WITH LIMITED PHAGOCYTICCAPACITY OF THE TARGET CELLS. THE ASSAY SYSTEM TO BE DEVELOPED WILL USE A FUNCTIONAL MOUSE MACROPHAGE CELL LINE. THIS CELL LINE, RAW264.7, IS AVIDLY PHAGOCYTIC, CAN UNDERGO A RESPIRATORY BURST, AND GROWS READILY IN CULTURE. THIS LATTER FEATURE WILL ALLOW BOTH CYTOTOXICITY AND GENOTOXICITY ENDPOINTS TO BE MEASURED. THE PHASE I GOALS ARE TO: (1) ESTABLISH THE OPTIMAL CONDITIONS FOR A CYTOTOXICITY ASSAY, (2) VALIDATE THE CYTOTOXICITY ASSAY WITH KNOWN TOXIC PARTICULATES, (3) ESTABLISH THE ASSAY CONDITIONS FOR AN HGPRT-BASED FORWARD MUTATION ASSAY, AND (4) ASSAY SEVERAL KNOWN CLASSES OF MUTAGENIC PARTICULATES.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government