Automated Neuron Tracing and 3D Reconstruction Software

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$1,569,255.00
Award Year:
2005
Program:
SBIR
Phase:
Phase II
Contract:
4R44MH072063-02
Award Id:
71747
Agency Tracking Number:
MH072063
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Microbrightfield, Inc., 185 Allen Brook Ln, Ste 201, Williston, VT, -
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
JACOB GLASER
(802) 288-9290
JGLASER@MICROBRIGHTFIELD.COM
Business Contact:
JACOB GLASER
(802) 288-9290
JGLASER@MICROBRIGHTFIELD.COM
Research Institution:
n/a
Abstract
DESCRIPTION (provided by applicant): We propose development of a commercial software product, AutoNeuron TM to perform automatic 3D neuron reconstruction. This software will run on a standard desktop PC with the Microsoft Windows(r) operating system and is performed on images acquired from specimen slides. Multiple image modalities can be used, including confocal microscopy, brightfield, and widefield fluorescence. A major innovation of this project is that AutoNeuron will operate in both interactive and unattended modes. Unattended automated tracing of many neuron images will be possible with our robust tracing algorithm based on edge detection and conduit tracking; however, some images will contain complexities that make automated tracing difficult. In these cases, the scientist marks branch points in interactive mode, while the software automatically traces the segment between marked points - accurately locating the centerline and tracing the diameter of the process along its length. In Phase I, we prove the feasibility of applying an advanced tracing algorithm to the larger problem of neuron reconstruction. During this phase we will develop a preliminary user interface with basic editing tools to be used in testing. The software accuracy and efficiency will be assessed comparatively with manual tracings from a variety of neuron types, staining, and imaging methods. Once feasibility of the tracing engine is established, a commercial product will be developed in Phase II. Neuron reconstruction and tracing are important aspects of many analyses of form and function in the nervous system. However, today many valuable studies are not considered because current manual and computer assisted reconstruction techniques are simply too laborious. An automated reconstruction workstation will improve the efficiency and productivity of many currently funded studies as well as future studies once considered impractical.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government