MEASUREMENT OF FAST ENZYME KINETICS VIA DROPLET MERGING

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1991
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
16619
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Microfab Technologies, Inc
1104 Summit Avenue, Plano, TX, 75074
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Wallace, David B
Principal Investigator
(214) 578-8076
Business Contact:
1 R43 GM44461-01A1
() -
Research Institution:
n/a
Abstract
THE USE OF HIGH SPEED (1-10M/S) 25-200FM DIAMETER DROPLETS MERGING IN FLIGHT WILL BE USED TO MEASURE ENZYME KINETICS IN THE 50FS-1MS RANGE. TWO EXTREMELY REPEATABLE DROPLET STREAMS ARE PRODUCED USING TECHNIQUES DEVELOPED FOR INK-JET PRINTING SYSTEMS. THE TWO DROPLET STREAMS MERGE AND A REACTION WILL TAKE PLACE ALMOST INSTANTANEOUSLY. THE ENZYME REACTION CAUSES CHANGES IN ABSORPTION, FLUORESCENCE, OR CHEMILUMINESCENCE, DEPENDING ON THE ENZYME AND SUBSTRATE USED IN THE REACTION. IN PHASE I OF THIS STUDY, CHEMILUMINESCENT REACTIONS WILL BE EVALUATED. THE PHOTONS EMITTED ARE DETECTED BY A PHOTOMULTIPLIER TUBE FOCUSED ON A REGION OF THE MERGED DROPLET STREAM. THE REGION CORRESPONDS TO THE LENGTH A DROPLET WOULD TRAVEL IN 1MS. THE DETECTOR IS GATED TO MEASURE EMISSIONS FOR @FS-1MS. (WITH 1FS RESOLUTION) AND THE GATE IS SYNCHRONIZED WITH THE DROPLET CREATION FREQUENCY, ALLOWING THE EMISSIONS FROM THOUSANDS OF DROPS TO BE INTEGRATED. THE SMALL DIAMETER OF THE DROPLETS INSURES RAPID MIXING. THE REPEATABILITY OF DROPLET FORMATION INHERENT WITH INK-JET TECHNOLOGY ALLOWS PHOTONS TO BE GATHERED OVER LONG TIME PERIODS, THUS INCREASING THE SENSITIVITY OF THE MEASUREMENT.

* information listed above is at the time of submission.

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