MEASUREMENT OF FAST ENZYME KINETICS VIA DROPLET MERGING

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 16619
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1991
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
1104 Summit Avenue, Plano, TX, 75074
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Wallace, David B
 Principal Investigator
 (214) 578-8076
Business Contact
 1 R43 GM44461-01A1
Phone: () -
Research Institution
N/A
Abstract
THE USE OF HIGH SPEED (1-10M/S) 25-200FM DIAMETER DROPLETS MERGING IN FLIGHT WILL BE USED TO MEASURE ENZYME KINETICS IN THE 50FS-1MS RANGE. TWO EXTREMELY REPEATABLE DROPLET STREAMS ARE PRODUCED USING TECHNIQUES DEVELOPED FOR INK-JET PRINTING SYSTEMS. THE TWO DROPLET STREAMS MERGE AND A REACTION WILL TAKE PLACE ALMOST INSTANTANEOUSLY. THE ENZYME REACTION CAUSES CHANGES IN ABSORPTION, FLUORESCENCE, OR CHEMILUMINESCENCE, DEPENDING ON THE ENZYME AND SUBSTRATE USED IN THE REACTION. IN PHASE I OF THIS STUDY, CHEMILUMINESCENT REACTIONS WILL BE EVALUATED. THE PHOTONS EMITTED ARE DETECTED BY A PHOTOMULTIPLIER TUBE FOCUSED ON A REGION OF THE MERGED DROPLET STREAM. THE REGION CORRESPONDS TO THE LENGTH A DROPLET WOULD TRAVEL IN 1MS. THE DETECTOR IS GATED TO MEASURE EMISSIONS FOR @FS-1MS. (WITH 1FS RESOLUTION) AND THE GATE IS SYNCHRONIZED WITH THE DROPLET CREATION FREQUENCY, ALLOWING THE EMISSIONS FROM THOUSANDS OF DROPS TO BE INTEGRATED. THE SMALL DIAMETER OF THE DROPLETS INSURES RAPID MIXING. THE REPEATABILITY OF DROPLET FORMATION INHERENT WITH INK-JET TECHNOLOGY ALLOWS PHOTONS TO BE GATHERED OVER LONG TIME PERIODS, THUS INCREASING THE SENSITIVITY OF THE MEASUREMENT.

* Information listed above is at the time of submission. *

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