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LC Microdispenser for High Resolution MALDI-TOF Analysis

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 2R44RR014939-02A2
Agency Tracking Number: RR014939
Amount: $346,570.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Solicitation Year: N/A
Award Year: 2003
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
PLANO, TX 75074
United States
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 (972) 578-8076
Business Contact
Phone: (972) 578-8076
Research Institution

DESCRIPTION (provided by applicant): We will construct a LC Microdispenser system for rapid liquid chromatography and high throughput loading of digested protein samples onto MALDI-TOF MS targets. This system will incorporate automated preparation of the reversed phase column, including automatic sample loading and integration into the microdispensing device. The capacity of the multi-fluid print head will be increased to 20 different fluids to increase throughput for multiple samples and/or to allow for different chromatography treatments. This system will provide for the maximum conservation of sample volume combined with the rapid LC separation of peptides, resulting in improved MALDI-TOF MS analysis through a reduction in ion suppression. The function and sensitivity of the LC Microdispensor system will be verified by the detection and peptide mass fingerprinting of low abundance proteins in Saccharomyces cerevisiae. Commercialization efforts will be conducted in parallel with the research efforts, in collaboration with our commercialization partners: Proteome Systems, Ltd., Shimadzu Biotech, and Millipore Corporation.

Commercial Applications:
High throughput instrumentation that improves the sensitivity and dynamic range during MALDI-TOF MS and results in the peptide mass fingerprinting of low abundance proteins is highly desired in the rapidly developing field of proteomics. Details regarding protein expression and post-translational modifications can reveal processes within a biological system useful for drug discovery and the diagnosis and treatment of disease and cancer.

* Information listed above is at the time of submission. *

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