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Micro-Western Blot for Proteomics Studies

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R43RR021792-01
Agency Tracking Number: RR021792
Amount: $100,000.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: PHS2006-2
Solicitation Year: 2006
Award Year: 2006
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
PLANO, TX 75074
United States
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 (972) 578-8076
Business Contact
Phone: (972) 578-8076
Research Institution

DESCRIPTION (provided by applicant): We will develop a system for the microdeposition of multiple phosphorylation site-specific antibodies onto protein bands of 1D or spots of 2D SDS-PAGE blots in the performance of protein phosphorylation studies. We will design and build a low volume loading print head for microdeposition of up to 10 different phosphospecific antibodies, or other fluids, pipette loaded at 3-5uL per channel. We will demonstrate the use of the low volume print head to deposit phosphospecific antibodies onto non-muscle Myosin Heavy Chain (MHC) bands in a Western blot to investigate the serine and threonine phosphorylation of MHC in mast cells during the antigen-induced secretory process. This new method has the significant advantage in examining for the presence and specific phosphorylation status of many proteins contained within one protein blot. This process will be more rapid, consume significantly less antibody, require a fewer number of gels and blots, and conserve precious protein samples. In circumstances where a protein sample is limited or the source of the antibody is rare or expensive, the Micro-Western blot method will provide a powerful alternative to conventional Western blotting methods.

* Information listed above is at the time of submission. *

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