Ink-Jet System for Protein Crystallization

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$849,804.00
Award Year:
2007
Program:
SBIR
Phase:
Phase II
Contract:
2R44CA105883-02A1
Award Id:
75557
Agency Tracking Number:
CA105883
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
MICROFAB TECHNOLOGIES, INC., 1104 SUMMIT AVE, STE 110, PLANO, TX, 75074
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
144937919
Principal Investigator:
PATRICK COOLEY
(972) 578-8076
PATRICK.COOLEY@MICROFAB.COM
Business Contact:
PATRICK COOLEY
() -
david.wallace@microfab.com
Research Institute:
n/a
Abstract
DESCRIPTION (provided by applicant): In this project, MicroFab will design and fabricate a prototype ink-jet based protein crystal screening platform (CrystalJet(tm)) with emphasis on membrane proteins that are difficult to crystallize, but critical to cel lular function. The prototype platform will perform high-throughput initial protein crystallization screening to identify parameters for subsequent scale-up to diffraction quality crystals. Information for computational and structure based drug development is typically obtained through x-ray diffraction studies of crystallized membrane proteins. However, the crystallization of membrane proteins often fails to produce diffraction quality crystals. Difficulties in crystallizing membrane proteins can be attrib uted to narrow metastable solubility regions and scarcity of purified protein. A solution to these problems is to decrease protein consumption and narrow the sampling of crystal growth conditions by using smaller screening volumes. Ink-jet dispensing is a technology capable of reliably delivering small volumes ranging from picoliter to microliter, the latter by accretion. The CrystalJet(tm) screening platform will operate in microbatch and vapor diffusion modes to offer experimental flexibility. We will als o develop a disposable ink-jet dispenser to address cross contamination issues. A dip-n- sip mode will be developed for the deposition of protein and matching volumes of crystallization/precipitant solution at volumes of lt1000 nL. A Cartesian fluid handli ng system will manipulate larger fluid volumes of gt1000 nL, such as oil and well precipitant solutions. The platform will include a plate handler and plate sealer to manipulate and seal crystal-screening plates, environmental control to maintain temperatu re, humidity and clean air, and a crystal observation and image capture system. A series of screening experiments will be performed using protein standards (e.g., lysozyme, glucose isomerase, thaumatin) and membrane proteins to validate the operation of th e CrystalJet(tm) platform. The CrystalJet platform is an automated tool to perform a greater number of protein crystallography screening experiments, while using significantly less protein in comparison to conventional screening systems. Ultimately, this t ool will enable faster and more efficient drug target discovery to aid in the development of effective drugs to treat disease.

* information listed above is at the time of submission.

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