BACULOVIRUS RECOMBINANTS THAT EXPRESS HEPATITIS B VIRUS SURFACE AN TIGEN: DEMONSTRATION FOR PRODUCTION OF SUBUNIT VACCINES

Award Information
Agency:
Department of Defense
Branch
Army
Amount:
$548,054.00
Award Year:
1987
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
1956
Agency Tracking Number:
1956
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
400 Frontage Rd, W Haven, CT, 06516
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Dr Mark A Cochran
(203) 932-3203
Business Contact:
() -
Research Institute:
n/a
Abstract
RECENTLY IT HAS BEEN DEMONSTRATED THAT BACULOVIRUSES CAN BE USED AS HIGH EFFICIENCY EUKARYOTIC CLONING AND EXPRESSION VECTORS FOR THE PRODUCTION OF FOREIGN PROTEINS. WE PROPOSE TO DEMONSTRATE THE FEASIBILITY AND BENEFITS OF USING THE BACULOVIRUS EXPRESSION SYSTEM FOR PRODUCTION OF SUBUNIT VACCINES. THIS DEMONSTRATION WILL BE EXEMPLIFIED BY THE EXPRESSION OF THE HBSAG-GENE IN INSECT CELLS INFECTED WITH RECOMBINANT BACULOVIRUS. SPECIAL ATTENTION WILL BE PAID TO THE TIME INVOLVED AND COST-EFFECTIVENESS OF OBTAINING THE FINAL PRODUCT. THIS DEMONSTRATION WILL INVOLVE THE FOLLOWING TASKS: CONSTRUCTION OF A RECOMBINANT BACULOVIRUS WHICH CONTAINS THE CODING SEQUENCE FOR HEPATITIS B VIRUS SURFACE ANTIGEN (HGSAG) UNDER THE CONTROL OF A BACULOVIRUS POLYHEDRIN-GENE PROMOTER. BIOCHEMICAL AND IMMUNOLOGICAL CHARACTERIZATION OF THE HBSAG GENERATED BY RECOMBINANT VIRUS IN INFECTED INVERTEBRATE TISSUE CULTURE CELLS AND CELL MEDIA. ANALYSIS OF STABILITY OF RECOMBINANT VIRUS DURING SEVERAL VIRUS GENEREATIONS. PILOT STUDY OF SCALED UP PRODUCTION IN SPINNER FLASKS.

* information listed above is at the time of submission.

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