MICROPHOTOHEMOLYSIS TO MEASURE ERYTHROCYTE FRAGILITY

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 16539
Amount: $49,856.00
Phase: Phase I
Program: SBIR
Awards Year: 1991
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Micro-med, Inc
707 Lyndon Lane, Suite 2, Louisville, KY, 40222
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Miller, Frederick N
 Principal Investigator
 (502) 588-7562
Business Contact
 1 R43 DK44057-01
Phone: () -
Research Institution
N/A
Abstract
ERYTHROCYTE MEMBRANE FRAGILITY MAY BE ALTERED BY DISEASE, AGE, AND DRUGS AND PLAYS AN IMPORTANT ROLE IN THE SURVIVAL AND FUNCTION OF THE ERYTHROCYTE. THE ONLY COMMONLY USED TECHNIQUE FOR ASSESSING THIS PARAMETER, THE OSMOTIC FRAGILITY TEST, IS VERY TIME CONSUMING, GIVES AN END-POINT ANALYSIS AND REQUIRES CONSIDERABLE EXPERTISE TO AVOID MANY SOURCES OF ERROR (PH, DILUTIONS, OSMOLALITY, CONTROL CELLS). USING ANIMAL ERYTHROCYTES WE HAVE DEVELOPED A TECHNIQUE, MICROPHOTOHEMOLYSIS, WHICH IS RAPID AND EASY TO USE AND GIVES BOTH THE RATE AND MAGNITUDE OF HEMOLYSIS WITH VERY SMALL VARIANCES. LIGHT THROUGH A MICROSCOPE IS USED FOR ACTIVATION OF A FLUOROCHROME-ERYTHROCYTE MIXTURE AND THE RESULTING HEMOLYSIS IN THE FIELD OF VIEW IS QUANTITATED BY A DETERMINATION OF THE INTENSITY OF TRANSMITTED LIGHT PASSING THROUGH THE SAMPLE. IN PHASE I, OUR AIMS ARE TO DETERMINE IF THIS TECHNIQUE CAN BE USED TO DISTINGUISH 1) NORMAL HUMAN ERYTHROCYTES FROM THOSE OF PATIENTS WITH SICKLE CELL ANEMIA OR HEREDITARY SPHEROCYTOSIS, 2) NORMAL CELLS AND FROM THOSE THAT HAVE BEEN TREATED WITH DRUGS WHICH ALTER MEMBRANE FRAGILITY AND, 3) SUBPOPULATIONS OF SICKLE CELLS OR OF DRUG TREATED NORMAL CELLS WITHIN THE SAME SAMPLE. OUR LONG TERM GOAL IS THE DEVELOPMENT OF AUTOMATED EQUIPMENT WHICH CAN BE USED TO SCREEN FOR DISEASE OR DRUG TREATMENTS AFFECTING ERYTHROCYTES.

* information listed above is at the time of submission.

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government