IN MAN

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1986
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
4808
Agency Tracking Number:
4808
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
1725 220th Street Se #104, Bothell, WA, 98021
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
DENNIS E SCHWARTZ
PRINCIPAL INVESTIGATOR
(206) 485-8566
Business Contact:
() -
Research Institute:
n/a
Abstract
IN THIS PROJECT, SYNTHETIC SHORT DNA PROBES WILL BE DEVELOPED FOR THE DIAGNOSIS OF SPECIFIC TYPES OF HUMAN PAPILLOMAVIRUS (HPV) INFECTIONS. SHORT DNA PROBES OF 20 TO 30 NUCLEOTIDES HAVE THE DESIRABLE PROPERTIES OF HIGH SPECIFICITY, FAST REACTION KINETICS, POTENTIAL HIGH SENSITIVITY, AND THE ABILITY TO BE SYNTHESIZED RAPIDLY IN LARGE AMOUNTS AT LOW COST. THEIR INHERENT HIGH SPECIFICITY IS AN IMPORTANT ATTRIBUTE. IT IS POSSIBLE TO DESIGN IN SITUHYBRIDIZATION ASSAYS THAT CAN DETECT AND DISCRIMINATE ANY OFTHE HPV TYPES PRESENTLY IDENTIFIED. WITH SHORT DNA PROBES, THE RESEARCHERS WILL DEMONSTRATE THAT A UNIVERSAL FORMAT FORIN SITU HYBRIDIZATION CAN BE DEVELOPED USING 2.4 M TETRA- ETHYLAMMONIUM CHLORIDE (TEACL) IN THE HYBRIDIZATION OR WASH STEPS. THIS SALT SOLUTION ALLOWS HYBRIDIZATION AND HYBRID MELTING REACTIONS TO OCCUR SOLELY AS A FUNCTION OF PROBE LENGTH. HENCE, SHORT PROBES OF THE SAME LENGTH (E.G., 25 MERS) WILL HAVE THE SAME HYBRIDIZATION AND MELTING PROPERTIES. IN ADDITION, THE PROJECT WILL DEMONSTRATE THAT AGITATION OF THE HYBRIDIZATION AND WASH STEPS WITH ULTRA- SOUND WILL SIGNIFICANTLY DECREASE THE TIME REQUIRED FOR THE IN SITU DETECTION OF THE VARIOUS TYPES OF HPV FROM THE 24 TO48 HOURS NOW REQUIRED. THE USE OF SHORT PROBES IN CONJUNCTION WITH TEACL SALTS AND ULTRASOUND IS EXPECTED TO BE IMPORTANT IN THE COMMERCIALIZATION OF DNA PROBE TECHNOLOGY FOR USE IN CLINICAL DIAGNOSIS.

* information listed above is at the time of submission.

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