A DNA PROBE FOR THE DIAGNOSIS OF CAMPYLOBACTER PYLORIDIS GASTRITIS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$487,000.00
Award Year:
1988
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Agency Tracking Number:
4889
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Microprobe Corp.
1725 220th Street Southease, Suite 104, Bothell, WA, 98021
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Dennis E Schwartz
Principal Investigator
(206) 485-8566
Business Contact:
() -
Research Institution:
n/a
Abstract
THE ROLE OF A SPECIFIC BACTERIUM AS THE POSSIBLE ETIOLOGIC AGENT OF GASTRITIS OR PEPTIC ULCER IS SUPPORTED BY A GROWINGSCIENTIFIC LITERATURE. HOWEVER, THE DIFFICULTY OF CONVENT- IONAL SAMPLING AND DIAGNOSTIC TECHNIQUES HAS RESTRICTED ADVANCES IN DISEASE DIAGNOSIS. THE PURPOSE OF THIS PHASE I PROJECT IS TO DEVELOP A DNA PROBE FROM THE CHROMOSOMAL DNA OF CAMPYLOBACTER PYLORIDIS, A MICROORGANISM RECENTLY SHOWN TO BE ASSOCIATED WITH GASTRITIS AND PEPTIC ULCER. THE 32P- LABELED PROBE WILL BE HYBRIDIZED TO CULTURED BACTERIAL DNA IMMOBILIZED ON NITROCELLULOSE. HYBRIDIZATION SPECIFICITY WILL BE EVALUATED IN A STANDARD NACL SOLUTION AND IN A NEW SALT SOLUTION, TETRAETHYLAMMONIUM CHLORIDE (TEACL). THE TEACL SYSTEM ABOLISHES THE PREFERENTIAL MELTING OF A-T VS. G-C BASE PAIRS IN DNA HYBRIDS, YIELDING A SIGNIFICANT SHARP-ENING OF THE MELTING PROFILE (<1C IN TEACL VS. 5-15C IN NACL), THUS ENHANCING PROBE SPECIFICITY. A DNA PROBE IN A TEACL SALT SYSTEM ALLOWS THE DEVELOPMENT OF A SENSITIVE AND SPECIFIC DNA PROBE ASSAY. THE LONG-TERM OBJECTIVE OF THE PROJECT IS TO PROVIDE A SIMPLE, RAPID, ACCURATE, AND LOW- COST METHOD FOR THE DETECTION OF CAMPYLOBACTER PYLORIDIS IN CLINICAL ISOLATES FROM PATIENTS WITH GASTRITIS OR PEPTIC ULCER. A DIAGNOSTIC TEST WILL BE PRODUCED COMMERCIALLY FOR BY CLINICAL DIAGNOSTIC LABORATORIES.

* information listed above is at the time of submission.

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