A DNA PROBE FOR THE DIAGNOSIS OF CAMPYLOBACTER PYLORIDIS GASTRITIS

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 4889
Amount: $487,000.00
Phase: Phase II
Program: SBIR
Awards Year: 1988
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Microprobe Corp.
1725 220th Street Southease, Suite 104, Bothell, WA, 98021
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Dennis E Schwartz
 Principal Investigator
 (206) 485-8566
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE ROLE OF A SPECIFIC BACTERIUM AS THE POSSIBLE ETIOLOGIC AGENT OF GASTRITIS OR PEPTIC ULCER IS SUPPORTED BY A GROWINGSCIENTIFIC LITERATURE. HOWEVER, THE DIFFICULTY OF CONVENT- IONAL SAMPLING AND DIAGNOSTIC TECHNIQUES HAS RESTRICTED ADVANCES IN DISEASE DIAGNOSIS. THE PURPOSE OF THIS PHASE I PROJECT IS TO DEVELOP A DNA PROBE FROM THE CHROMOSOMAL DNA OF CAMPYLOBACTER PYLORIDIS, A MICROORGANISM RECENTLY SHOWN TO BE ASSOCIATED WITH GASTRITIS AND PEPTIC ULCER. THE 32P- LABELED PROBE WILL BE HYBRIDIZED TO CULTURED BACTERIAL DNA IMMOBILIZED ON NITROCELLULOSE. HYBRIDIZATION SPECIFICITY WILL BE EVALUATED IN A STANDARD NACL SOLUTION AND IN A NEW SALT SOLUTION, TETRAETHYLAMMONIUM CHLORIDE (TEACL). THE TEACL SYSTEM ABOLISHES THE PREFERENTIAL MELTING OF A-T VS. G-C BASE PAIRS IN DNA HYBRIDS, YIELDING A SIGNIFICANT SHARP-ENING OF THE MELTING PROFILE (<1C IN TEACL VS. 5-15C IN NACL), THUS ENHANCING PROBE SPECIFICITY. A DNA PROBE IN A TEACL SALT SYSTEM ALLOWS THE DEVELOPMENT OF A SENSITIVE AND SPECIFIC DNA PROBE ASSAY. THE LONG-TERM OBJECTIVE OF THE PROJECT IS TO PROVIDE A SIMPLE, RAPID, ACCURATE, AND LOW- COST METHOD FOR THE DETECTION OF CAMPYLOBACTER PYLORIDIS IN CLINICAL ISOLATES FROM PATIENTS WITH GASTRITIS OR PEPTIC ULCER. A DIAGNOSTIC TEST WILL BE PRODUCED COMMERCIALLY FOR BY CLINICAL DIAGNOSTIC LABORATORIES.

* information listed above is at the time of submission.

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