SYNTHETIC OLIGONUCLEOTIDES FOR TRYPANOSOME CHEMOTHERAPY

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$490,442.00
Award Year:
1989
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
7094
Agency Tracking Number:
7094
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
1725 220th St Southeast #104, Bothell, WA, 98021
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Dennis E Schwartz Phd
(206) 485-8566
Business Contact:
() -
Research Institute:
n/a
Abstract
THE 5' ENDS OF ALL TRYPANOSOME MRNAS HAVE RECENTLY BEEN SHOWN TO HAVE A COMMON 35-NUCLEOTIDE SPLICED LEADER (SL). THE SL IS BELIEVED TO ARISE BY TRANSCRIPTION WITHIN A MINI-EXON-DERIVED RNA (MEDRNA) OF ABOUT 140 NUCLEOTIDES, FOLLOWED BY ITS TRANS-SPLICING WITH THE PRIMARY TRANSCRIPTS OF MRNAS. THE PRESENCE OF THE SL IN ALL TRYPANOSOMAL MRNAS PROVIDES ANIDEAL SYSTEM TO TEST THE EFFICACY OF SHORT OLIGONUCLEOTIDES AS THERAPEUTIC AGENTS. SYNTHETIC OLIGONUCLEOTIDES, CONSISTING OF DIESTERS, ETHYL PHOSPHOTRIESTERS, OR METHYL PHOSPHONATES, WILL BE SYNTHESIZED. THESE WILL BE COMPLEMENTARY TO THE SL SEQUENCE OR TO THE SPLICE-DONOR SITE OF THE MEDRNA TO DIRECTLY INHIBIT PROTEIN SYNTHESIS OR MRNA MATURATION. THESE OLIGONUCLEOTIDES WILL BE TESTED FOR THEIR ABILITY TO INHIBIT PROTEIN SYNTHESIS IN CELL-FREE SYSTEMS USING PURIFIED MRNA, AND IN CELL CULTURES OF TRYPANOSOMA BRUCEI. SPECIFIC ANTISENSE OLIGONUCLEOTIDES WILL PROVIDE VALUABLE CHEMOTHERAPEUTIC AGENTS FOR THE TREATMENT OF SIGNIFICANT HUMAN DISEASES.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government