RETROVIRUS MODEL--EVUALUATE ANTI-SENSE PROBE THERAPY

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1989
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
11414
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Microprobe Corp
1725 220th St Se 104, Bothell, WA, 98021
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
VIRAL OR PLASMID CONSTRUCTS CONTAINING A HUMAN GROWTH HORMONE (HGH) GENE POSITIONED DOWNSTREAM FROM AN AUTHENTIC VIRAL OR HOST TRANSCRIPTIONAL CONTROL SEQUENCE SHOULD PROVIDE A CONVENIENT SYSTEM IN WHICH TO RAPIDLY AND QUANTITATIVELY EVALUATE ANTISENSE OLIGONUCLEOTIDES DIRECTED TOWARD LEADER RNA. PHASE I WORK WILL USE A PROVIRAL VECTOR DERIVED FROM A MODIFIED ROUS SARCOMA VIRUS CONTAINING THE HGH GENE SUBSTITUTED FOR THE SRC GENE. VIRAL INFECTIONS WILL BE ESTABLISHED IN CELL CULTURE USING THIS VECTOR, AND THE EFFECTS OF ANTISENSE OLIGONUCLEOTIDES ON RNA PROCESSING WILL BE MONITORED BY ASSAYING FOR HGH GENE EXPRESSION. OLIGONUCLEOTIDES OF VARIOUS LENGTHS, CONTAINING EITHER NORMAL PHOSPHODIESTER OR METHYLPHOSPHONATE LINKAGES, WILL BEEVALUATED FOR ANTISENSE ACTIVITY. THE ADDITION OF TERMINAL ACRIDINE RESIDUES TO THE OLIGONUCLEOTIDES WILL ALSO BE EVALUATED FOR THEIR EFFECT ON ANTISENSE ACTIVITY. DEMONSTRATION OF THE EFFICACY OF THIS APPROACH WILL LEAD TO A PHASE II EFFORT IN WHICH A HUMAN IMMUNODEFICIENCY VIRUS(HIV)-DIRECTED HGH GENE EXPRESSION SYSTEM WILL BE CONSTRUCTED FOR EVALUATION OF ANTISENSE PROBES PRIOR TO USE IN AN HIV-INFECTED CELL LINE.

* information listed above is at the time of submission.

Agency Micro-sites

US Flag An Official Website of the United States Government