DNA PROBE TEST TO DETECT ORAL CANDIDA IN AIDS PATIENTS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$49,988.00
Award Year:
1990
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
13636
Agency Tracking Number:
13636
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
1725 220th St Se #104, Bothell, WA, 98021
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Diana K Sheiness
(206) 485-8566
Business Contact:
() -
Research Institution:
n/a
Abstract
THIS RESEARCH WILL PROVIDE THE FOUNDATION FOR A RAPID, INEXPENSIVE, NON-CULTURE-BASED TEST FOR THE QUANTITATIVE DETECTION OF ORAL CANDIDA ALBICANS IN HIV-POSITIVE PATIENTS. THE PRIMARY EXPERIMENTAL OBJECTIVE IS TO CREATE A SET OF SHORT SYNTHETIC OLIGODEOXYNUCLEOTIDE PROBES THAT WILL HYBRIDIZE SPECIFICALLY WITH THE 16S RRNA OF C. ALBICANS. SPECIFICITY WILL BE VERIFIED BY HYBRIDIZATION OF EACH (32)P-LABELED PROBE TO SLOT BLOT FILTERS CONTAINING NUCLEIC ACID SAMPLES FROM OVER 50 MICROORGANISMS THAT ARE FOUND FREQUENTLY IN THE OROPHARYNX. FURTHERMORE, THE (32)-LABELEDPROBES WILL BE USED TO MEASURE LEVELS OF C. ALBICANS IN SAMPLES FROM IMMUNOCOMPROMISED PATIENTS. PROBE RESULTS WILL BE COMPARED WITH CONVENTIONAL DIAGNOSTIC EVALUATIONS OF CANDIDIASIS IN THESE SAME PATIENTS. DURING PHASE II OF THIS PROJECT, THESE PROBES WILL BE USED TO DEVELOP A RAPID COLORIMETRIC ASSAY FOR DETECTING AND MONITORING C. ALBICANS IN PERSONS AT HIGH RISK FOR DEVELOPING ACQUIRED IMMUNODEFICIENCY SYNDROME (AIDS). THE FINAL PRODUCT WILL BE A QUANTITATIVE TEST FOR THE CLINICAL LABORATORY THAT CAN BE PERFORMED WITHIN 3 HOURS OR LESS AT ROOM TEMPERATURE AND THAT CAN BE USED AS AN ALTERNATIVE FOR THE MUCH LENGTHIER PROCEDURE OF CULTURING FOR C. ALBICANS.

* information listed above is at the time of submission.

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