HIV REPORTER CELL LINE FOR SCREENING ANTI-SENSE AGENTS

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 16307
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1991
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Microprobe Corpon
1725 220th Street Se, #104, Bothell, WA, 98021
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Gamper, Howard B
 Principal Investigator
 (206) 485-8566
Business Contact
 1 R43 AI31315-01
Phone: () -
Research Institution
N/A
Abstract
A SECRETABLE ALKALINE PHOSPHATASE (SPAP) REPORTER GENE WILL BE SUBSTITUTED FOR A PORTION OF THE ENV GENE IN AN HIV PROVIRAL DNA CLONE. EXPRESSION OF THE REPORTER GENE WILL BE REGULATED BY A COMPLETE COMPLEMENT OF VIRAL CIS AND TRANS ACTING CONTROL ELEMENTS, SUCH THAT THE LEVEL OF EXTRACELLULAR ALKALINE PHOSPHATASE WILL SERVE AS AN INDICATOR OF VIRAL GAG, POL, AND ENV REGIONS SUBJECT TO NORMAL REGULATION BY THE VIRAL CODED TAT, REV, AND NEF GENE PRODUCTS. THE H9 CELL LINE WILL BE TRANSFECTED WITH THIS CONSTRUCT AND THE TRANSIENT EXPRESSION OF SPAP, P24, AND REVERSE TRANSCRIPTASE CHARACTERIZED. THE FEASIBILITY OF USING THE SPAP AS A MARKER TO DETECT ANTIVIRAL ACTIVITY WILL BE ASSESSED BY MONITORING THE EFFECT OF THREE ANTISENSE OLIGONUCLEOTIDES (ODNS) TARGETED TO CRITICAL SITES ON THE HIV GENOME IN THE TRANSFECTED H9 CELLS.

* information listed above is at the time of submission.

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