DNA PROBES TO DETECT PREDISPOSITION TO TYPE I DIABETES

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$470,000.00
Award Year:
1994
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Agency Tracking Number:
19209
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Microprobe Corp.
1725 220th Street Se 104, Bothell, WA, 98021
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Nicolaas M J Vermeulen
(800) 447-4362
Business Contact:
() -
Research Institution:
n/a
Abstract
INSULIN-DEPENDENT DIABETES MELLITUS (TYPE I) OCCURS IN ABOUT 0.5% OF CAUCASIANS. THE INCIDENCE INCREASES OVER 20-FOLD IN INDIVIDUALS WITH THE SPEIFIC SUSCEPTIBILITY GENE HLA-DQ3.2. APPROXIMATELY 70% OF INDIVIDUALS WHO BECOME INSULIN-DEPENDENT DIABETICS CARRY THIS GENE, MAKING IT THE MOST SPECIFIC MARKER KNOWN FOR IDENTIFYING INDIVIDUALS AT RISK. CLEARLY THIS ILLUSTRATES THE NEED FOR SCREENING AND IDENTIFICATION OF THE DQ3.2 GENE. IN THIS WORK, DNA OLIGONUCLEOTIDE PROBES WILL BE USED IN A NYLON BEAD-BASED SANDWICH HYBRIDIZATION ASSAY FOR THE DETECTION OF THE DQ3.2 GENE IN HUMAN LYMPHOCYTES. HYBRIDIZATION WITH TARGET DQ3.2 GENE WILL BE DETECTED USING A CHEMILUMINESCENT SIGNAL SYSTEM. THE PROCEDURES WILL BE DEVELOPED FOR THE RAPID EXTRACTION AND DETECTION OF THE DQ3.2 GENE WITH HIGH SPECIFICITY AND SENSITIVITY.

* information listed above is at the time of submission.

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