DNA Probe Detection of Oral Spirochetes

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1 R43 DE11218-01A1,
Agency Tracking Number: 29351
Amount: $98,307.00
Phase: Phase I
Program: SBIR
Awards Year: 1995
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Microprobe Corp.
1725 220th Street SE, 104, Bothell, WA, 98021
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Paul Haydock
 () -
Business Contact
Phone: () -
Research Institution
Treponema denticola is an oral spirochete associated with periodontal disease. T. denticola belongsfamily of oral spirochetes which, although found in low numbers in healthy tissue, increases in numbOral treponemes have the ability to invade gingival and connective tissues by mechanisms which are nunderstood. Since routine culture of these organisms is difficult, the most commonly used method ofpresence of oral spirochetes is dark field microscopy of wet mounts, a method not readily adaptablepractice. It is our aim to develop DNA probes directed to T. denticola, the newly identified PathogeSprochete (PROS), and other oral spirochetes and to incorporate these probes into a semi-automated aIn Phase I of this project, DNA probes will be developed and tested. In Phase II, the assay incorporbe characterized and tested in actual patient populations to determine which probe is most informatipatient care. This assay will ultimately provide a simple chair-side means of detecting the presenceallowing for patient risk assessment and treatment efficacy.

* information listed above is at the time of submission.

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