ORIENTED PROTEIN MICROARRAY FOR FUNCTIONAL STUDIES

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$100,000.00
Award Year:
2002
Program:
SBIR
Phase:
Phase I
Contract:
1R43RR017130-01
Award Id:
60104
Agency Tracking Number:
RR017130
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
MICROSURFACES, INC., 4220 CENTRAL AVE NE, STE 101, MINNEAPOLIS, MN, 55421
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
ATHENA GUO
(763) 788-0255
ATHENA@MEMSURFACE.COM
Business Contact:
ATHENA GUO
(763) 788-0255
ATHENA@MEMSURFACE.COM
Research Institute:
n/a
Abstract
DESCRIPTION (provided by applicant):A novel method is proposed for the fabrication of protein microarrays using metal coordination chemistry and poly-histidine tagged protein molecules. The proposed method is aimed at microarrays with highly selective and facile linking chemistry, with uniform and controllable orientation of immobilized protein molecules, and with proteins in native states and easily accessible by targets in the solution. Preliminary experiments using 6x-His terminated green-fluorescence protein (GFP) demonstrated the feasibility and potential of the proposed approach. We will establish the advantage of an oriented protein microarray in protein-ligand interaction studies over randomly oriented arrays fabricated by existing methods. We also propose to explore protein immobilization without purification on the highly selective metal ion surface. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

* information listed above is at the time of submission.

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