CONTRIBUTION OF SURFACE PROPERTIES TO ANTIBODY IMMOBILIZATION

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 4941
Amount: $239,000.00
Phase: Phase II
Program: SBIR
Awards Year: 1987
Solitcitation Year: N/A
Solitcitation Topic Code: N/A
Solitcitation Number: N/A
Small Business Information
Midwest Research Microscopy
5510 West Florist Avenue, Milwaukee, WI, 53218
Duns: N/A
Hubzone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Susan J Mroczkowski
 Principal Investigator
 (414) 527-2260
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE IMMOBILIZATION OF PROTEINS ON INSOLUBLE SURFACES IS IMPORTANT IN DIAGNOSTIC INSTRUMENTATION AND IN VIVO MONITOR-ING. IMPROVEMENTS IN THE SURFACE ANTIBODY INTERACTIONS SHOULD RESULT IN MORE EFFICIENT APPROACHES TO IMMUNO- DIAGNOSTICS. THIS WOULD BE ESPECIALLY USEFUL IN THE FIELD OF TUMOR MARKER DETECTION WHERE PANELS OF ANTIGENS MAY BE NEEDED FOR PROPER DIAGNOSIS. THIS RESEARCH IS THE DEVELOPMENT OF A MULTIPLE IMMUNOLOGICAL TEST WITH AN ELECTRONIC ENDPOINT. THE RESEARCH WILL INVOLVE THE USE OF ANTIGEN OR ANTIBODY-LABELED COLLOIDAL GOLD PARTICLES TO GENERATE AN ELECTRONIC ENDPOINT. A DIAGNOSTIC ELEMENT WILL BE REFINED SO THAT THE ANTIBODY-ANTIGEN REACTION CAUSES THE COMPLETION OF AN OPEN ELECTRICAL CIRCUIT. THIS MODEL NECESSITATES AN ELECTRICALLY INSULATING BASE SURFACE FOR PROTEIN ADHESION. A PAIR OF CONDUCTORS IS PLACED ON THE DEFINING A CHANNEL BETWEEN THEM. THE ANTIGEN IS PLACED IN THE CHANNEL. THE GOLD-TAGGED ANTIBODY WILL BOND TO THE SPECIFIC ANTIGEN, FORMING A CONDUCTIVE BRIDGE ACROSS THE CHANNEL FOR A POSITIVE REACTION. THIS NEW TYPE OF DEVICE COULD REPLACE THE CURRENTLY USED ENZYME IMMUNOASSAY, IMMUNO-FLUORESCENCE, AND RADIOIMMUNOASSAY TECHNIQUES, WHICH ARE RELIABLE BUT SLOW, TEDIOUS, AND RESTRICTED TO SINGLE TESTS PER SAMPLE. THE COMMERCIAL APPLICATIONS OF A MULTIPLE TEST ENCOMPASS HUMAN DIAGNOSTICS SUCH AS CANCER AND ALLERGY AS WELL AS VETERINARY AND FORENSIC DIAGNOSTICS.

* information listed above is at the time of submission.

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