CONTRIBUTION OF SURFACE PROPERTIES TO ANTIBODY IMMOBILIZATION

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 4941
Amount: $239,000.00
Phase: Phase II
Program: SBIR
Awards Year: 1987
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Midwest Research Microscopy
5510 West Florist Avenue, Milwaukee, WI, 53218
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Susan J Mroczkowski
 Principal Investigator
 (414) 527-2260
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE IMMOBILIZATION OF PROTEINS ON INSOLUBLE SURFACES IS IMPORTANT IN DIAGNOSTIC INSTRUMENTATION AND IN VIVO MONITOR-ING. IMPROVEMENTS IN THE SURFACE ANTIBODY INTERACTIONS SHOULD RESULT IN MORE EFFICIENT APPROACHES TO IMMUNO- DIAGNOSTICS. THIS WOULD BE ESPECIALLY USEFUL IN THE FIELD OF TUMOR MARKER DETECTION WHERE PANELS OF ANTIGENS MAY BE NEEDED FOR PROPER DIAGNOSIS. THIS RESEARCH IS THE DEVELOPMENT OF A MULTIPLE IMMUNOLOGICAL TEST WITH AN ELECTRONIC ENDPOINT. THE RESEARCH WILL INVOLVE THE USE OF ANTIGEN OR ANTIBODY-LABELED COLLOIDAL GOLD PARTICLES TO GENERATE AN ELECTRONIC ENDPOINT. A DIAGNOSTIC ELEMENT WILL BE REFINED SO THAT THE ANTIBODY-ANTIGEN REACTION CAUSES THE COMPLETION OF AN OPEN ELECTRICAL CIRCUIT. THIS MODEL NECESSITATES AN ELECTRICALLY INSULATING BASE SURFACE FOR PROTEIN ADHESION. A PAIR OF CONDUCTORS IS PLACED ON THE DEFINING A CHANNEL BETWEEN THEM. THE ANTIGEN IS PLACED IN THE CHANNEL. THE GOLD-TAGGED ANTIBODY WILL BOND TO THE SPECIFIC ANTIGEN, FORMING A CONDUCTIVE BRIDGE ACROSS THE CHANNEL FOR A POSITIVE REACTION. THIS NEW TYPE OF DEVICE COULD REPLACE THE CURRENTLY USED ENZYME IMMUNOASSAY, IMMUNO-FLUORESCENCE, AND RADIOIMMUNOASSAY TECHNIQUES, WHICH ARE RELIABLE BUT SLOW, TEDIOUS, AND RESTRICTED TO SINGLE TESTS PER SAMPLE. THE COMMERCIAL APPLICATIONS OF A MULTIPLE TEST ENCOMPASS HUMAN DIAGNOSTICS SUCH AS CANCER AND ALLERGY AS WELL AS VETERINARY AND FORENSIC DIAGNOSTICS.

* information listed above is at the time of submission.

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government