LARGE-SCALE PRODUCTION OF AVIAN MYELOBLASTOSIS VIRUS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$41,000.00
Award Year:
1987
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
7157
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Molecular Biology Resources
5520 West Burleigh Street, Milwaukee, WI, 53210
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
RICHARD F PIEHL PHD
(414) 871-7199
Business Contact:
() -
Research Institution:
n/a
Abstract
AVIAN MYELOBLASTOSIS VIRUS (AMV), A SOURCE OF REVERSE TRANSCRIPTASE FOR USE IN RECOMBINANT DNA WORK, IS CURRENTLY PRODUCED COMMERCIALLY FROM THE PLASMA OF LEUKEMIC CHICKS. PRODUCTION INVOLVES INOCULATION OF SEVERAL HUNDRED DAY-OLD CHICKS WITH AMV AND HARVEST OF VIRUS-CONTAINING BLOOD BY HEART PUNCTURE 7 TO 10 DAYS LATER. THE RESEARCH PROPOSED WILL INVESTIGATE THE FEASIBILITY OF COMMERCIAL-SCALE PRODUCTION OF AMV FROM CELL CULTURES OF CHICKEN MYELOBLASTS.PROCEDURES FOR IN VITRO TRANSFORMATION OF CHICKEN MACROPHAGELINEAGE CELLS WITH AMV AND THE IN VITRO GROWTH OF THE TRANSFORMED CELLS ON A SMALL SCALE ARE WELL KNOWN. LARGE-SCALE CULTURE OF AMV-TRANSFORMED MYELOBLASTS HAS ALSO BEEN ACHIEVED, BUT WITH SPECIALLY CONSTRUCTED APPARATUS. THE PRESENT PROPOSAL DISCUSSES ADAPTATION OF RECENT ADVANCESIN CELL CULTURE CONDITIONS AND APPARATUS FOR LARGE-SCALE CELL CULTURE TO PRODUCTION OF AMV. SUCCESSFUL DEVELOPMENT OF THE CELL CULTURE METHOD WILL GIVE A PROCEDURE THAT IS BOTH MORE ECONOMICAL AND MORE HUMANE THAN THE PRESENT IN VIVO METHOD.

* information listed above is at the time of submission.

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