LARGE-SCALE PRODUCTION OF AVIAN MYELOBLASTOSIS VIRUS

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 7157
Amount: $41,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1987
Solitcitation Year: N/A
Solitcitation Topic Code: N/A
Solitcitation Number: N/A
Small Business Information
Molecular Biology Resources
5520 West Burleigh Street, Milwaukee, WI, 53210
Duns: N/A
Hubzone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 RICHARD F PIEHL PHD
 (414) 871-7199
Business Contact
Phone: () -
Research Institution
N/A
Abstract
AVIAN MYELOBLASTOSIS VIRUS (AMV), A SOURCE OF REVERSE TRANSCRIPTASE FOR USE IN RECOMBINANT DNA WORK, IS CURRENTLY PRODUCED COMMERCIALLY FROM THE PLASMA OF LEUKEMIC CHICKS. PRODUCTION INVOLVES INOCULATION OF SEVERAL HUNDRED DAY-OLD CHICKS WITH AMV AND HARVEST OF VIRUS-CONTAINING BLOOD BY HEART PUNCTURE 7 TO 10 DAYS LATER. THE RESEARCH PROPOSED WILL INVESTIGATE THE FEASIBILITY OF COMMERCIAL-SCALE PRODUCTION OF AMV FROM CELL CULTURES OF CHICKEN MYELOBLASTS.PROCEDURES FOR IN VITRO TRANSFORMATION OF CHICKEN MACROPHAGELINEAGE CELLS WITH AMV AND THE IN VITRO GROWTH OF THE TRANSFORMED CELLS ON A SMALL SCALE ARE WELL KNOWN. LARGE-SCALE CULTURE OF AMV-TRANSFORMED MYELOBLASTS HAS ALSO BEEN ACHIEVED, BUT WITH SPECIALLY CONSTRUCTED APPARATUS. THE PRESENT PROPOSAL DISCUSSES ADAPTATION OF RECENT ADVANCESIN CELL CULTURE CONDITIONS AND APPARATUS FOR LARGE-SCALE CELL CULTURE TO PRODUCTION OF AMV. SUCCESSFUL DEVELOPMENT OF THE CELL CULTURE METHOD WILL GIVE A PROCEDURE THAT IS BOTH MORE ECONOMICAL AND MORE HUMANE THAN THE PRESENT IN VIVO METHOD.

* information listed above is at the time of submission.

Agency Micro-sites

US Flag An Official Website of the United States Government