MAGNETIC MICROSPHERE DEPLETION OF T-LYMPHOCYTES

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$165,000.00
Award Year:
1986
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
1412
Agency Tracking Number:
1412
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
11180-a Roselle Street, San Diego, CA, 92121
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Kenneth J. Widder
Executive Officer
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
THIS RESEARCH IS DIRECTED TOWARD DEVELOPING MEANS OF UTILIZING MAGNETIC MICROSPHERES TO RAPIDLY AND RELIABLY SEPARATE TARGET CELLS. INITIALLY THE TARGET CELL WILL BE PERIPHERAL BLOOD T-LYMPHOCYTES AND THE WORK WILL EVALUATE THE ABILITY TO DEPLETE THIS CELL POPULATION. LATER, THE SYSTEM WILL BE APPLIED TO BONE MARROW TOWARD THE GOAL OF ELIMINATING GRAFT VERSUS HOST DISEASE (GVHD). REMOVAL OF T-LYMPHOCYTES FROM DONOR MARROW HAS BEEN SHOWN TO GREATLY REDUCE OR ELIMINATE GVHD. PHASE I WILL STUDY TWO DIFFERENT MAGNETIC MICROSPHERES COUPLED TO TWO DIFFERENT ANTIBODIES BY VARIOUS MEANS FOR T-LYMPHOCYTE REMOVAL: T-LYMPHOCYTE REMOVAL, AS JUDGED BY E-ROSETTING AND FLUORESCENT TAGGING, WILL INDICATE WHICH MICROSPHERE-ANTIBODY COUPLING METHODS ARE THE MOST EFFICIENT AND COST-EFFECTIVE. PHASE II WILL TEST THE METHOD(S) SELECTED IN PHASE I FOR T-LYMPHOCYTES REMOVAL FROM NORMAL DONOR MARROW BY BIOLOGICAL ASSAYS AND E-ROSETTING AND FLUORESCENT TAGGING. THE COLONY FORMING ABILITY OF STEM CELLS WILL BE DETERMINED TO ASSESS MARROW VIABILITY. THE METHOD(S) OF CHOICE WILL THEN BE TESTED IN PATIENTS. THIS RESEARCH IS DESIGNED TO LEAD TO A METHOD FOR PREVENTING GVHD WHICH CAN BE USED IN A CLINICAL SETTING.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government