ROTAVIRUSES ARE THE MAJOR CAUSE OF SERIOUS INFANTILE DIARRHEA.

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1985
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
2918
Agency Tracking Number:
2918
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
11180a Roselle Street, San Diego, CA, 92121
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
ROBERT N. BRYAN
PRINCIPAL INVESTIGATOR
(619) 452-0681
Business Contact:
() -
Research Institution:
n/a
Abstract
ROTAVIRUSES ARE THE MAJOR CAUSE OF SERIOUS INFANTILE DIARRHEA. THIS PATHOGEN ACCOUNTS FOR 4060% OF THE DIAGNOSED CASES OF GASTROENTERITIS IN CHILDREN UNDER THE AGE OF TWO YEARS. DIAGNOSIS IS CURRENTLY MADE BY ELISA OR BY ELECTRON MICROSCOPY. THE LATTER IS IMPRACTICAL FOR THE SMALL LABORATORY, AND THE ELISA PRODUCES AN UNACCEPTABLE NUMBER OF FALSE POSITIVES. THE VIRUS CONSISTS OF 11 SEGMENTS OF DOUBLE-STRANDED RNA. THE NUCLEOTIDE SEQUENCE OF THE MAJOR OUTER CAPSID GLYCOPROTEIN GENE IS KNOWN FOR THREE HUMAN SEROTYPES. WE PROPOSE TO SYNTHESIZE DNA PROBES WHICH WILL HYBRIDIZE SPECIFICALLY WITH A REGION OF THE GENOME COMMON TO THE HUMAN SEROTYPES. PHASE I. BASED ON PUBLISHED SEQUENCE INFORMATION, WE WILL SYNTHESIZE AN OLIGONUCLEOTIDE PROBE COMPLEMENTARY BOTH TO THE PLUS STRAND RNA OF THE GENOME AND THE MRNA OF THE VIRUS. THE PROBE WILL BE TESTED FOR ITS ABILITY TO DETECT ROTAVIRUS IN CHARACTERIZED STOOL SAMPLES. THE MAJOR TECHNICAL INNOVATION IN THIS WORK WILL BE THE DEVELOPMENT OF METHODS WHICH ALLOW OLIGONUCLEOTIDE PROBES TO BE USED TO DETECT DOUBLE-STRANDED RNA VIRUSES IN CLINICAL SAMPLES. PHASE II. WE WILL CONDUCT CLINICAL TRIALS OF THE BEST PROBES SYNTHESIZED, PRIMARILY NONISOTOPICALLY LABELED, IN ORDER TO PRODUCE A CLINICAL DIAGNOSTIC KIT TO RAPIDLY DETECT ROTAVIRUS IN STOOL SAMPLES.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government