THE LONG-TERM GOAL OF THIS RESEARCH IS TO DEVELOP A HIGHLY SENSITIVE NONRADIOACTIVE DETECTION METHOD FOR DNA HYBRIDS WHICH WILL BE USEFUL WITH CLINICAL SAMPLES.

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 3201
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1985
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
11180a Roselle St., San Diego, CA, 92121
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 LYLE J. ARNOLD
 PRINCIPAL INVESTIGATOR
 (619) 452-0681
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE LONG-TERM GOAL OF THIS RESEARCH IS TO DEVELOP A HIGHLY SENSITIVE NONRADIOACTIVE DETECTION METHOD FOR DNA HYBRIDS WHICH WILL BE USEFUL WITH CLINICAL SAMPLES. DNA HYBRIDIZATION PROBES ARE PROVING TO BE USEFUL FOR THE DETECTION OF SPECIFIC BACTERIA AND VIRUSES AS WELL AS FOR THE DETECTION OF INHERITED OR ACQUIRED (SUCH AS ONCOGENES) GENETIC ALTERATIONS. IN ORDER TO FULLY UTILIZE THE POTENTIAL OF SUCH PROBES IN A CLINICAL SETTING A HIGHLY SENSITIVE NONRADIOACTIVE DETECTION METHOD IS NEEDED. CURRENTLY RADIOACTIVE DETECTION IS THE MOST SENSITIVE; HOWEVER, ENZYMES WITH A HIGH TURNOVER NUMBER THAT CAN BE COUPLED TO THE GENERATION OF LIGHT CAN GIVE EVEN GREATER SENSITIVITY. WE PROPOSE TO DEVELOP SUCH A SENSITIVE METHOD BY COUPLING THE PRODUCTION OF NADH BY GLUCOSE-6-PHOSPHATE DEHYDROGENASE TO BACTERIAL LUCIFERASE USING FMN:NADH OXIDOREDUCTASE. SINCE APPROPRIATE INSTRUMENTATION CAN DETECT LIGHT WITH EXTREME SENSITIVITY, SUCH A LIGHT-GENERATING SYSTEM CAN BE 102105 TIMES MORE SENSITIVE THAN CURRENT COLORIMETRIC METHODS AND 101102 TIMES MORE SENSITIVE THAN RADIOLABELING.

* Information listed above is at the time of submission. *

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