A MICROFLUORIMETRIC DNA HYBRIDIZATION ASSAY

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 3200
Amount: $500,000.00
Phase: Phase II
Program: SBIR
Awards Year: 1986
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Molecular Biosystems, Inc.
11180a Roselle Street, San Diego, CA, 92121
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Michael J. Heller
 Principal Investigator
 (619) 452-0681
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE GOAL OF THIS RESEARCH IS TO DEVELOP A RAPID AND HIGHLY SENSITIVE DIRECT FLUORESCENT DETECTION SYSTEM FOR DNA HYBRIDS. DNA HYBRIDIZATION PROBES ARE USEFUL FOR DETECTING SPECIFIC PATHOGENS AND GENETIC ALTERATIONS. RADIOAUTOGRAPHY, CURRENTLY THE MOST SENSITIVE DETECTION TECHNIQUE, CAN VISUALIZE 15 PICOGRAMS OF TARGET DNA IN AN OVERNIGHT EXPOSURE. IN ORDER TO FULLY UTILIZE THE POTENTIAL OF DNA PROBES IN A CLINICAL SETTING, A SIMPLE, RAPID, SENSITIVE, NONRADIOACTIVE DETECTION METHOD IS NEEDED. FLUORESCENCE DETECTION, WITH ITS HIGH INHERENT SENSITIVITY, SHOULD PROVIDE SUCH A SYSTEM. IN PHASE I WE PROPOSE TO INVESTIGATE A FLUORESCENT HYBRID DETECTION SYSTEM UTILIZING FLUORESCENT OLIGONUCLEOTIDE HYBRIDIZATION PROBES IN A FOCUSED HYBRIDIZATION FORMAT, IN COMBINATION WITH A HIGHLY SENSITIVE MICROFLUORIMETER. PRELIMINARY RESULTS SUGGEST THAT INSTANTANEOUS DETECTION LIMITS FOR SUCH A SYSTEM ARE AT A LEVEL PROMISING SUPERIOR PERFORMANCE AND UTILITY. IN PHASE II WE PROPOSE TO CONDUCT TRIALS OF THE SYSTEM FOR DETECTING VIRAL AND BACTERIAL DNA TARGETS IN CLINICAL SAMPLES, WITH THE GOAL OF PRODUCING A RAPID, SIMPLE, AND SPECIFIC DETECTION SYSTEM.

* information listed above is at the time of submission.

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