MOLECULAR DIAGNOSTICS FOR EPSTEIN-BARR VIRUS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$500,000.00
Award Year:
1988
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
2919
Agency Tracking Number:
2919
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
3100 Wyman Park Drive, Baltimore, MD, 21211
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Alan L. Scott
Principal Investigator
(301) 955-3442
Business Contact:
() -
Research Institute:
n/a
Abstract
EPSTEIN-BARR VIRUS (EBV) CAUSES MONONUCLEOSIS, IS STRONGLY IMPLICATED IN BURKITT'S LYMPHOMA AND NASOPHARYNGEAL CARCINOMA, AND MAY BE ASSOCIATED WITH RHEUMATOID ARTHRITIS. INDIVIDUALS WITH THESE DISEASES PRODUCE ANTIBODIES TO VIRAL EARLY ANTIGENS, CAPSID ANTIGENS, AND NUCLEAR ANTIGENS. THE IMMUNE RESPONSE TO EBV ANTIGENS HAS BEEN SHOWN TO BE AN IMPORTANT INDICATOR FOR CLINICAL EVALUATION AND TREATMENT. HOWEVER, CURRENT IMMUNE ASSAYS LACK SENSITIVITY AND SELECTIVITY, REQUIRE EXPERT INTERPRETATION, AND ARE NOT AMENABLE TO LARGE-SCALE CLINICAL STUDIES. OUR GOAL IS TO IMPROVE THE CLINICAL DIAGNOSTICS FOR EBV-RELATED DISEASES. GENES FOR EBV ANTIGENS WERE CLONED INTO A HIGH EXPRESSION PLASMID AND THE CORRESPONDING EBV POLYPEPTIDES WERE SYNTHESIZED IN BACTERIA, PURIFIED, AND USED TO DEVELOP SENSITIVE ENZYME-LINKED IMMUNOSORBENT ASSAYS (ELISA) IN THE PROPOSED RESEARCH, THESE ELISA METHODS WILL BE USED TO MEASURE CLASS-SPECIFIC (IGG, IGA, IGM) ANTIBODY TITERS TO EBV ANTIGENS IN NORMAL INDIVIDUALS AND IN PATIENTS WITH ACUTE AND CHRONIC MONONUCLEOSIS, BURKITT'S LYMPHOMA, NASOPHARYNGEAL CARCINOMA, AND RHEUMATOID ARTHRITIS. THE SPECIFIC ANTIBODY LEVELS WILL BE CORRELATED WITH CLINICAL DATA TO DETERMINE THE EFFICACY OF THE ELISA MEASUREMENTS AS PROGNOSTIC INDICATORS. IN THE INITIAL STUDIES OF PHASE I, THE ANALYSIS WILL BE LIMITED TO SAMPLES FROM SERUM BANKS. IF THE DATA SUPPORT THE CLINICAL VALUE OF THE ASSAYS, PHASE II STUDIES WILL ENTAIL FIELD TRIALS INVOLVING CLOSE COLLABORATION WITH CLINICIANS.

* information listed above is at the time of submission.

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