AFFINITY PURIFICATION OF PARAINFLUENZA VIRUS GLYCOPROTEINS

Award Information
Agency:
Department of Health and Human Services
Amount:
$498,100.00
Program:
SBIR
Contract:
N/A
Solitcitation Year:
N/A
Solicitation Number:
N/A
Branch:
N/A
Award Year:
1986
Phase:
Phase II
Agency Tracking Number:
2939
Solicitation Topic Code:
N/A
Small Business Information
Molecular Engineering Associat
2001 Park Place, Birmingham, AL, 35203
Hubzone Owned:
N
Woman Owned:
N
Socially and Economically Disadvantaged:
N
Duns:
N/A
Principal Investigator
 Ranjit Ray
 Principal Investigator
 (205) 934-5036
Business Contact
Phone: () -
Research Institution
N/A
Abstract
IT IS PROPOSED TO PURIFY THE TWO ENVELOPE GLYCOPROTEINS, HN AND F, FROM HUMAN PARAINFLUENZA TYPE 3 (PI3) VIRUS BY AFFINITY CHROMATOGRAPHY USING SPECIFIC MONOCLONAL ANTIBODIES AND TO INCORPORATE THEM INTO LIPID VESICLES. THE EXPERIMENTAL APPROACH INVOLVES IMMOBILIZATION OF SPECIFIC MONOCLONAL ANTIBODY ON A SEPHAROSE 4B MATRIX. PURIFIED VIRUS OR INFECTED CELLS WILL BE USED AS THE SOURCE OF ANTIGENS FOR ADSORPTION AND PURIFICATION OF SPECIFIC GLYCOPROTEINS THROUGH AFFINITY COLUMNS. THE TWO PURIFIED ANTIGENS WILL BE INCORPORATED IN DEFINED PROPORTIONS INTO LIPID VESICLES. THIS RESEARCH WILL FACILITATE THE DEVELOPMENT OF MORE EFFECTIVE VACCINES AND WILL LEAD TO A PHASE II PROPOSAL IN WHICH THE EFFICACY OF THE RECONSTITUTED GLYCOPROTEIN-CONTAINING VESICLES WILL BE EVALUATED AS A VACCINE IN EXPERIMENTAL ANIMALS.

* information listed above is at the time of submission.

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