NOVEL FLUOROGENIC ENZYME SUBSTRATES AND THEIR USES

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$495,678.00
Award Year:
1994
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
19282
Agency Tracking Number:
19282
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
4849 Pitchford Avenue, Eugene, OR, 97402
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Richard P Haugland
(503) 344-3007
Business Contact:
() -
Research Institute:
n/a
Abstract
THIS PROJECT INVOLVES DEVELOPMENT OF NOVEL FLUOROGENIC SUBSTRATES AND THEIR BIOANALYTICAL APPLICATIONS. SUPPORT IS SPECIFICALLY REQUESTED FOR SYNTHESIS OF FLUOROGENIC ENZYME SUBSTRATES THAT YIELD INTENSELY FLUORESCENT PRECIPITATES NEAR THE SITE OF ENZYMATIC ACTIVITY. WORK IN PROGRESS HAS DEMONSTRATED A SIGNIFICANT POTENTIAL FOR THESE REAGENTS FOR ULTRASENSITIVE DETECTION IN HISTOCHEMISTRY, MOLECULAR BIOLOGICAL TECHNIQUES INCLUDING DNA HYBRIDIZATION, DETECTION OF LOW ABUNDANCE CELLULAR RECEPTORS AND DETECTION OF ENZYMES IN LIVING CELLS. ENZYMATIC AMPLIFICATION OF THE SIGNAL RESULTS IN FLUORESCENCE THAT IS ORDERS OF MAGNITUDE GREATER THAN THAT POSSIBLE WITH FLUORESCENT DYE CONJUGATES AND SIGNIFICANTLY GREATER THAN THAT POSSIBLE WITH SUBSTRATES THAT YIELD COLORED PRECIPITATES. SINCE THE FLUORESCENT PRECIPITATES REPRESENT "PURE" DYE WITH NO CARRIER PROTEIN, THE SUBSTRATES CAN POTENTIALLY BE USED TO DETECT ENZYMATIC ACTIVITY WITH HIGH SPATIAL RESOLUTION WHETHER THE ENZYME IS INTRINSICALLY PRESENT IN CELLS OR IS USED AS A TOOL IN A CONJUGATED FORM SUCH AS IN ELISA TECHNIQUES.

* information listed above is at the time of submission.

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