NOVEL FLUOROGENIC ENZYME SUBSTRATES AND THEIR USES

Award Information
Agency:
Department of Health and Human Services
Amount:
$495,678.00
Program:
SBIR
Contract:
N/A
Solitcitation Year:
N/A
Solicitation Number:
N/A
Branch:
N/A
Award Year:
1994
Phase:
Phase II
Agency Tracking Number:
19282
Solicitation Topic Code:
N/A
Small Business Information
Molecular Probes Inc.
4849 Pitchford Avenue, Eugene, OR, 97402
Hubzone Owned:
N
Woman Owned:
N
Socially and Economically Disadvantaged:
N
Duns:
N/A
Principal Investigator
 Richard P Haugland
 (503) 344-3007
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THIS PROJECT INVOLVES DEVELOPMENT OF NOVEL FLUOROGENIC SUBSTRATES AND THEIR BIOANALYTICAL APPLICATIONS. SUPPORT IS SPECIFICALLY REQUESTED FOR SYNTHESIS OF FLUOROGENIC ENZYME SUBSTRATES THAT YIELD INTENSELY FLUORESCENT PRECIPITATES NEAR THE SITE OF ENZYMATIC ACTIVITY. WORK IN PROGRESS HAS DEMONSTRATED A SIGNIFICANT POTENTIAL FOR THESE REAGENTS FOR ULTRASENSITIVE DETECTION IN HISTOCHEMISTRY, MOLECULAR BIOLOGICAL TECHNIQUES INCLUDING DNA HYBRIDIZATION, DETECTION OF LOW ABUNDANCE CELLULAR RECEPTORS AND DETECTION OF ENZYMES IN LIVING CELLS. ENZYMATIC AMPLIFICATION OF THE SIGNAL RESULTS IN FLUORESCENCE THAT IS ORDERS OF MAGNITUDE GREATER THAN THAT POSSIBLE WITH FLUORESCENT DYE CONJUGATES AND SIGNIFICANTLY GREATER THAN THAT POSSIBLE WITH SUBSTRATES THAT YIELD COLORED PRECIPITATES. SINCE THE FLUORESCENT PRECIPITATES REPRESENT "PURE" DYE WITH NO CARRIER PROTEIN, THE SUBSTRATES CAN POTENTIALLY BE USED TO DETECT ENZYMATIC ACTIVITY WITH HIGH SPATIAL RESOLUTION WHETHER THE ENZYME IS INTRINSICALLY PRESENT IN CELLS OR IS USED AS A TOOL IN A CONJUGATED FORM SUCH AS IN ELISA TECHNIQUES.

* information listed above is at the time of submission.

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