ALTHOUGH TWO-DIMENSIONAL ELECTROPHORESIS HAS PROVEN TO BE A POWERFUL TECHNIQUE FOR SEPARATING AND IDENTIFYING PROTEINS IN COMPLEX MIXTURES, THE AVAILABILITY OF SUITABLE GRADIENT GELS LIMITS THE UTILITY OF THIS TECHNIQUE AND INVOLVES CONSIDERABLE

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1986
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
4943
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Moleculon Research Corp
230 Albany Street, Cambridge, MA, 02139
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
LARRY D NICHOLS
PRINCIPAL INVESTIGATOR
(617) 577-9900
Business Contact:
() -
Research Institution:
n/a
Abstract
ALTHOUGH TWO-DIMENSIONAL ELECTROPHORESIS HAS PROVEN TO BE A POWERFUL TECHNIQUE FOR SEPARATING AND IDENTIFYING PROTEINS IN COMPLEX MIXTURES, THE AVAILABILITY OF SUITABLE GRADIENT GELS LIMITS THE UTILITY OF THIS TECHNIQUE AND INVOLVES CONSIDERABLE TIME-CONSUMING HANDWORK. AN ADDITIONAL PROBLEMSTEMS FROM THE LACK OF ADEQUATE SEPARATION OF LOW MOLECULAR WEIGHT COMPOUNDS (<10,000 DALTONS). IT WOULD BE DESIRABLE TO PRODUCE A HIGH-CAPACITY PRECAST GRADIENT GEL SYSTEM THAT PERMITS ANALYSIS OF IMPORTANT PROTEINS AND PEPTIDES BELOW 10,000 DALTONS. POROPLASTIC GELS ARE WELL SUITED TO THIS AND WOULD OFFER THE FOLLOWING ADVANTAGES: LOWER MOLECULAR WEIGHT RANGE; UNIFORMITY AND REPRODUCIBILITY; EASE OF MANUFACTURE; POTENTIALLY BETTER RESOLUTION/SEPARATION; AND EASE OF VARYING SOLVENT. DURING THIS PHASE I PROGRAM, THREEDIFFERENT TECHNIQUES FOR PRODUCTION OF POROPLASTIC GRADIENT GELS WILL BE INVESTIGATED. THE POROPLASTIC GRADIENT GEL MEMBRANES PRODUCED WILL BE COMPARED TO COMMERCIALLY AVAILABLE POLYACRYLAMIDE GRADIENT GELS IN THEIR ABILITY TO SEPARATE VARIOUS PROTEIN MIXTURES WITH PARTICULAR EMPHASIS ON THE <10,000 DALTON RANGE.

* information listed above is at the time of submission.

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