MONOCLONAL ANTIBODIES (MABS) ARE BEING USED WITH EXPLOSIVE FREQUENCY AND IN EVER-INCREASING AMOUNTS IN BIOMEDICAL RESEARCH AND MAY PLAY AN IMPORTANT ROLE IN THE FUTURE DIAGNOSIS AND TREATMENT OF HUMAN DISEASES.

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1986
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
4697
Agency Tracking Number:
4697
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
15719 Crabbs Branch Way, Derwood, MD, 20855
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
FREDERICK J DARFLER
PRINCIPAL INVESTIGATOR
(301) 330-8162
Business Contact:
() -
Research Institute:
n/a
Abstract
MONOCLONAL ANTIBODIES (MABS) ARE BEING USED WITH EXPLOSIVE FREQUENCY AND IN EVER-INCREASING AMOUNTS IN BIOMEDICAL RESEARCH AND MAY PLAY AN IMPORTANT ROLE IN THE FUTURE DIAGNOSIS AND TREATMENT OF HUMAN DISEASES. THIS PROJECT PROPOSES: 1. TO DESIGN A SYSTEM FOR THE FORMATION OF HYBRIDOMAS SECRETING HUMAN MABS USING IN VITRO IMMUNIZATION;AND 2. TO DESIGN A METHOD TO GROW LARGE NUMBERS OF THESE HYBRIDOMAS IN VITRO. TO REDUCE THE PROBLEMS OF CONTAMINATION OF MAB PREPARATIONS BY MICROBES, ENDOTOXINS, AND FOREIGN PROTEINS, AND TO ENHANCE THE EFFICIENCY OF HYBRID FORMATION, ALL PHASES OF HYBRIDOMA GROWTH AND FORMATION WILL BE DONE IN A COMPLETELY SERUM-FREE DEFINED MEDIUM CONTAINING LESS THAN 15 MG/ML OF PROTEIN. IN VITRO IMMUNIZATION WILL BE CARRIED OUT WITH ALCOHOL-FIXED HUMAN B-LYMPHOMA CELLS AS ANTIGEN USING A TECHNIQUE THAT RESULTS IN THE SUCCESSFUL FORMATION OF MURINE BYDRIDS IN VITRO. LARGE-SCALE GROWTH IN SERUM-FREE MEDIUM OF ANTIGEN-SPECIFIC HYBRIDS WILL BE DONE USING LARGE SPINNER FLASKS. PURIFICATION OF MABS FROM THESE CULTURES WILL BE DONE BY ULTRAFILTRATION, AMMONIUM SULFATE PRECIPITATION, AND DIALYSIS, RESULTING IN PREPARATIONS OF OVER 90% PURITY AT MINIMAL COST.

* information listed above is at the time of submission.

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