Custom Oligonucleotide Libraries synthesis

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$214,713.00
Award Year:
2010
Program:
SBIR
Phase:
Phase I
Contract:
1R43GM093579-01
Award Id:
96221
Agency Tracking Number:
GM093579
Solicitation Year:
n/a
Solicitation Topic Code:
NIGMS
Solicitation Number:
n/a
Small Business Information
BIODISCOVERY, LLC, 5692 Plymouth rd, ANN ARBOR, MI, 48105
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
611643813
Principal Investigator:
JEANMARIEROUILLARD
(734) 763-4722
JMROUILL@UMICH.EDU
Business Contact:
MARIEROUILLARD
() -
jmr@biodiscovery-llc.com
Research Institute:
n/a
Abstract
DESCRIPTION (provided by applicant): There is a growing demand for custom synthesis of short genes libraries coding for active peptides or regulatory RNAs. DNA microarrays can be manufactured by synthesizing oligonucleotides on solid substrate in a mas sively-parallel manner using a high-yield low cost chemistry. Oligonucleotides can be cleaved off the microarray surface and recovered as a pool. Our hypothesis is that we can use this technology to create custom libraries of long DNA oligonucleotides at a much reduced cost and increased complexity compared to current technologies. Our long term objectives are to implement a commercial service of affordable custom synthesis of long oligonucleotide libraries. These libraries are used as a research tools in m any applications such as studies on gene silencing, protein-DNA interaction, epitope mapping or even antimicrobial peptides. There are no limits for applications than the imagination of scientists. The heath relatedness of the project resides in the facts that these applications lead to the discovery of new cellular mechanisms, diagnosis tools, drugs or even vaccines. The scope of the proposed project is 1) to demonstrate the feasibility of using an emulsion-based PCR to amplify oligonucleotide libraries; 2) to investigate the possibility to synthesize libraries of oligonucleotide up to 150 mer in length and 3) to determine the synthesis error rate and type of sequence mutations present in these libraries. We will in particular test the effect of droplet si ze and number of templates per droplet on the PCR amplification of oligonucleotide template in an emulsion. We wil characterize the complexity of an amplified library by deep-sequencing a PCR product. The large amount of sequence information obtained will also permit an in depth characterization the type of errors occuring during massively-parallel long oligonucleotide synthesis. PUBLIC HEALTH RELEVANCE: The unprecedented availability of affordable custom libraries of long oligonucleotides will e nable new experimentations in fields such as gene silencing, protein-DNA interaction, epitope mapping or even antimicrobial peptides. This technology will undoubtedly bolster the discovery of new cellular mechanisms, diagnosis tools, drugs or even vaccines , ultimately benefiting the society.

* information listed above is at the time of submission.

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