High Efficiency Transfection via Nanospearing
Small Business Information
NANOLAB, INC., 55 CHAPEL ST, NEWTON, MA, 02458
AbstractDESCRIPTION (provided by applicant): NanoLab is proposing to develop a new biomolecule delivery technique named nanospearing , based on its previously published work. DNA plasmids are immobilized on magnetically susceptible, needle-like nanostructures. Th ese needles can be magnetically accelerated to penetrate ('spear') the cell membrane. The plasmid DNA bound to the nanotubes can be released and expressed in the cells. Two pieces of equipment, called GeneBlender and GeneHammer, are proposed for spearing c ells in suspension and on substrates respectively. Theoretical analysis is to to be used for optimizing the system configuration so that nanotubes can be driven to their highest speed for spearing. Different molecular immobilization strategies will be test ed to achieve the efficient plasmid loading. Upon ressolving the potential pitfalls, the process will be universalized and packaged into a transfection kit. The development will be focused on plasmid DNA delivery at this phase. It is enasaged for applicati ons in vivo, such as drug delivery and vaccination.
* information listed above is at the time of submission.