High Efficiency Transfection via Nanospearing

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$398,332.00
Award Year:
2007
Program:
SBIR
Phase:
Phase I
Contract:
1R43EB006249-01A1
Award Id:
85596
Agency Tracking Number:
EB006249
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
NANOLAB, INC., 55 CHAPEL ST, NEWTON, MA, 02458
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
153008631
Principal Investigator:
DONG CAI
(617) 347-3728
CAID@BC.EDU
Business Contact:
DAVID CARNAHAN
() -
dcarnahan@nano-lab.com
Research Institute:
n/a
Abstract
DESCRIPTION (provided by applicant): NanoLab is proposing to develop a new biomolecule delivery technique named nanospearing , based on its previously published work. DNA plasmids are immobilized on magnetically susceptible, needle-like nanostructures. Th ese needles can be magnetically accelerated to penetrate ('spear') the cell membrane. The plasmid DNA bound to the nanotubes can be released and expressed in the cells. Two pieces of equipment, called GeneBlender and GeneHammer, are proposed for spearing c ells in suspension and on substrates respectively. Theoretical analysis is to to be used for optimizing the system configuration so that nanotubes can be driven to their highest speed for spearing. Different molecular immobilization strategies will be test ed to achieve the efficient plasmid loading. Upon ressolving the potential pitfalls, the process will be universalized and packaged into a transfection kit. The development will be focused on plasmid DNA delivery at this phase. It is enasaged for applicati ons in vivo, such as drug delivery and vaccination.

* information listed above is at the time of submission.

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