DEVELOPMENT OF AN ULTRASENSITIVE DETECTION SYSTEM FOR DNA SEQUENCING

Award Information
Agency:
Department of Energy
Branch
n/a
Amount:
$49,867.00
Award Year:
1991
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
14536
Agency Tracking Number:
14536
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Life Sciences Building Rm 008, State University Of New York, Stony Brook, NY, 11794
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Dr Frederic R Furuya
Principal Investigator
() -
Business Contact:
(516) 282-3372
Research Institute:
n/a
Abstract
A NEW GOLD PARTICLE HAS BEEN DEVELOPED THAT IS WATER SOLUBLE, CAN BE REACTED WITH NUCLEIC ACIDS IN A SPECIFIC WAY, AND IS HIGHLY SENSITIVE TO SILVER DEVELOPER. THIS SYSTEM HAS EXPERIMENTALLY DEMONSTRATED POTENTIAL TO DETECT 10(-20) MOL OF TARGET. IN PHASE I, BOTH THE COUPLING OF THEGOLD PARTICLE TO NUCLEIC ACIDS AND THE SILVER DEVELOPMENT ISBEING OPTIMIZED. THE LIMIT OF SENSITIVITY AFTER AMPLIFICATION WILL THEN BE DETERMINED. THE GOLD PARTICLE ISBEING APPLIED TO MODEL DNA ASSAY SYSTEMS THAT REQUIRE HIGH SENSITIVITY AND HIGH SPECIFICITY; THE PARTICLE IS THEN AMPLIFIED WITH SILVER DEVELOPER TO EVALUATE ITS PERFORMANCE AS A NUCLEIC ACID PROBE. THE CAPABILITY OF THE GOLD PARTICLE SYSTEM IS BEING EVALUATED IN HYBRIDIZATION ASSAYS AND IN GELS TO DETERMINE WHICH METHOD IS BETTER FOR FRAGMENT DETECTION. ITS PERFORMANCE IN MODELS FOR MULTIPLEXSEQUENCING IS ALSO BEING EXAMINED. THESE TESTS PROVIDE A BASIS FOR EVALUATION OF ITS USE IN STRATEGIES FOR MORE RAPID SEQUENCING RATES THAT INCLUDE SEQUENCING BY HYBRIDIZATION AND MULTIPLEX SEQUENCING.

* information listed above is at the time of submission.

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