AMPLIFICATION OF SILVER-GOLD IMMUNO- AND DNA PROBES

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 16433
Amount: $49,838.00
Phase: Phase I
Program: SBIR
Awards Year: 1991
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Life Sciences Building, Room 0, 08 Suny @stony Brook, Stony Brook, NY, 11794
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Powell, Richard D
 Principal Investigator
 (516) 632-9235
Business Contact
 1 R43 CA55432-01
Phone: () -
Research Institution
N/A
Abstract
NEW METHODOLOGIES WILL BE USED FOR THE DETECTION AND QUANTITIZATION OF VERY SMALL AMOUNTS OF PROTEINS AND DNA SEQUENCES. A NEW GOLD PARTICLE WILL BE USED TO LABEL TARGET BIOMOLECULES, THEN STAINED WITH METALLIC SILVER BY EXPOSURE TO AQUEOUS SILVER (I) IONS AND A REDUCING AGENT. THE SILVER WILL THEN BE EXTRACTED AND DETERMINED IN AQUEOUS SOLUTION, USING DYE COUPLING REACTIONS AT A POLYMER SURFACE IN CONJUNCTION WITH SPECTROPHOTOMETRIC MEASUREMENTS. COLORIMETRIC ANALYSIS, CATALYTIC SPECTROPHOTOMETRIC MEASUREMENTS AND ANODIC STRIPPING VOLTAMMETRY WILL ALSO BE USED TO DEVELOP FAST, SIMPLE AMPLIFICATION PROCEDURES FOR THE SILVER-STAINED GOLD LABEL. CALCULATIONS INDICATE THAT SENSITIVITIES OVER 10,000 TIMES GREATER THAN THOSE CURRENTLY AVAILABLE CAN BE ACHIEVED: THIS WOULD ALLOW THE DETECTION OF 1 X 10 TO THE -23 MOLE OF ANTIBODY OR DNA (EQUIVALENT TO 8 X 10 TO THE -19 G OF ALKALINE PHOSPHATASE, OR 1.2 X 10 TO THE -20 G OF A FOUR-BASE OLIGONUCLEOTIDE).

* Information listed above is at the time of submission. *

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