AMPLIFICATION OF SILVER-GOLD IMMUNO- AND DNA PROBES

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$49,838.00
Award Year:
1991
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
16433
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Nanoprobes, Inc
Life Sciences Building, Room 0, 08 Suny @stony Brook, Stony Brook, NY, 11794
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Powell, Richard D
Principal Investigator
(516) 632-9235
Business Contact:
1 R43 CA55432-01
() -
Research Institution:
n/a
Abstract
NEW METHODOLOGIES WILL BE USED FOR THE DETECTION AND QUANTITIZATION OF VERY SMALL AMOUNTS OF PROTEINS AND DNA SEQUENCES. A NEW GOLD PARTICLE WILL BE USED TO LABEL TARGET BIOMOLECULES, THEN STAINED WITH METALLIC SILVER BY EXPOSURE TO AQUEOUS SILVER (I) IONS AND A REDUCING AGENT. THE SILVER WILL THEN BE EXTRACTED AND DETERMINED IN AQUEOUS SOLUTION, USING DYE COUPLING REACTIONS AT A POLYMER SURFACE IN CONJUNCTION WITH SPECTROPHOTOMETRIC MEASUREMENTS. COLORIMETRIC ANALYSIS, CATALYTIC SPECTROPHOTOMETRIC MEASUREMENTS AND ANODIC STRIPPING VOLTAMMETRY WILL ALSO BE USED TO DEVELOP FAST, SIMPLE AMPLIFICATION PROCEDURES FOR THE SILVER-STAINED GOLD LABEL. CALCULATIONS INDICATE THAT SENSITIVITIES OVER 10,000 TIMES GREATER THAN THOSE CURRENTLY AVAILABLE CAN BE ACHIEVED: THIS WOULD ALLOW THE DETECTION OF 1 X 10 TO THE -23 MOLE OF ANTIBODY OR DNA (EQUIVALENT TO 8 X 10 TO THE -19 G OF ALKALINE PHOSPHATASE, OR 1.2 X 10 TO THE -20 G OF A FOUR-BASE OLIGONUCLEOTIDE).

* information listed above is at the time of submission.

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