MOLECULAR CYTOSKELETAL MICROSCOPY PROBES AND BIOLOGICAL WIRES

Award Information
Agency: National Science Foundation
Branch: N/A
Contract: N/A
Agency Tracking Number: 27402
Amount: $64,993.00
Phase: Phase I
Program: SBIR
Awards Year: 1994
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Nanoprobes Inc.
25 E Loop Rd, Stony Brook, NY, 11790
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Richard D Powell
 (516) 444-8815
Business Contact
Phone: () -
Research Institution
N/A
Abstract
NOVEL PROBES ARE INTRODUCED FOR THE STUDY OF CYTOSKELETAL STRUCTURES AND PROCESSES BY ELECTRON MICROSCOPY. THESE COMPRISE LARGE CLUSTER COMPLEXES OF GOLD, PLANTINUM, AND PALLADIUM 0.8 NM-2.4 NM IN DIAMETER COVALENTLY LINKED TO PHALLOIDIN, CYTOCHALASIN B, COLCHICINE, AND TAXOL VIA A SINGLE REACTIVE FUNCTIONAL GROUP INCORPORATED INTO THEIR LIGAND SPHERES. THE CONJUGATES CAN PENETRATE 20-30 MUM OR MORE INTO CELL SECTIONS AND ACHIEVE SIGNIFICANTLY HIGHER RESOLUTION THAN COLLOIDAL GOLD-ANTIBODY CONJUGATES. THEY ARE MICROINJECTABLE; COMPATIBLE WITH LIVING CELLS; AND USED TO EXTEND THE USE OF ELECTRON MICROSCOPY TO THE STUDY OF ORGANISMS SUCH AS PROCARYOTIC BACTERIA, IN WHICH ANTIBODY PROBES ARE NOT FEASIBLE. COMBINED LABELS INCLUDING BOTH A GOLD CLUSTER AND A FLUORESCENT MOIETY ARE BEING USED TO PREPARE ADDITIONAL PROBES WITH THE SAME MOLECULES, ENABLING CORRELATION OF FLUORESCENCE CONFOCAL MICROSCOPY AND ELECTRON MICROSCOPY DATA FROM A SINGLE LABELING EXPERIMENT. THE PREPARATION OF MOLECULAR WIRES IS BEING ATTEMPTED BY THE IN VITRO ASSEMBLY OF GOLD-LABELED CYTOSKELETAL COMPONENTS, FOLLOWED BY SILVER ENHANCEMENT.

* information listed above is at the time of submission.

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