PARALYTIC SHELLFISH POISONING: NEW METHODS FOR ASSAY AND DEP

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 3146
Amount: $355,000.00
Phase: Phase II
Program: SBIR
Awards Year: 1986
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Neushul Mariculture, Inc.
475 Kellogg Way, Goleta, CA, 93117
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Bruce W. W. Harger
 Principal Investigator
 (805) 964-5844
Business Contact
Phone: () -
Research Institution
N/A
Abstract
PARALYTIC SHELLFISH POISONING (PSP) IS A GROWING PUBLIC HEALTH PROBLEM. THE PRESENCE OF TOXINS IN SHELLFISH IS RATHER CRUDELY MEASURED BY INJECTING SHELLFISH EXTRACTS INTO MICE, AND RECORDING THE TIME THAT ELAPSES BETWEEN INJECTION AND DEATH. WE PROPOSE TO DEVELOP AN EASILY USED ASSAY TO DETERMINE THE PRESENCE OF TOXINS IN SHELLFISH AND USE BIOCHEMICAL AND PHYSIOLOGICAL APPROACHES IN DEVISING METHODS FOR SHELLFISH DETOXIFICATION. SHELLFISH THAT ARE PRESENTLY BEING GROWN EXPERIMENTALLY IN THE SEA BY NEUSHUL MARICULTURE INCORPORATED (NMI) ACCUMULATE TOXIN, BUT THE LEVEL OF TOXICITY PRODUCED IS NOT UNIFORM. THEREFORE, TOXIC DINOFLAGELLATES WILL BE CULTURED IN THE LABORATORY, AND FED TO SHELLFISH IN CONTROLLED CONCENTRATIONS TO PRODUCE UNIFORMLY TOXIC SHELLFISH. THE TOXIN LEVELS IN THESE SHELLFISH WILL BE MEASURED USING THE STANDARD MOUSE TEST AND THE EFFECT OF THE TOXIN ON TISSUE CULTURES WILL ALSO BE MEASURED. WE WILL ALSO TEST SOLUTION FLUOROMETRIC TECHNIQUES AS ASSAY METHODS. THE PROPOSED RESEARCH PROJECT WOULD MEET SOME OF THE OBJECTIVES OF THE NATIONAL ORGANIZATIONS CONCERNED WITH THE EFFECTS OF PARALYTIC SHELLFISH POISONING INCLUDING THE SHELLFISH SANITATION BRANCH OF THE FOOD AND DRUG ADMINISTRATION, THE NATIONAL CENTER FOR TOXICOLOGICAL RESEARCH OF THE FDA, AND THE NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES.

* information listed above is at the time of submission.

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