Ruminant B-Lymphocyte Yellow Fluorescent Protein Aggregation Bioassay for Elk Chronic Wasting Disease

Award Information
Agency:
Department of Defense
Branch
Army
Amount:
$100,000.00
Award Year:
2004
Program:
STTR
Phase:
Phase I
Contract:
W81XWH-04-C-0141
Award Id:
68463
Agency Tracking Number:
A045-027-0237
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
1024 S. Innovation Way, Stillwater, OK, 74074
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
879735579
Principal Investigator:
David Goad
Principal Investigator
(405) 372-9535
dgoad@nomadics.com
Business Contact:
James Luby
Chief Operating Officer
(405) 372-9535
jluby@nomadics.com
Research Institute:
Oklahoma State University
Kenneth Clinkenbeard
College of Veterinary Medicine, 221 McElroy Hall
Stillwater, OK, 74078
(405) 744-4467
Nonprofit college or university
Abstract
The goal of the proposed research is to develop a cell culture model for elk chronic wasting disease (CWD) prion propagation that can be used as a bioassay for detecting CWD. Our goal will be accomplished by bioengineering a bovine B-lymphocyte cell (B-cell) line to surface express elk PrPc fused to yellow fluorescent protein (YFP). Interaction of these B-cells with infectious, mis-folded, protease resistant CWD prion (PrPcwd) will induce conversion of the dispersed YFP-PrPc to aggregated mis-folded YFP-PrPcwd that will be detected by confocal microscopy as aggregated YFP-PrPcwd on the B-cell surface. Once Phase I proof-of-concept has been demonstrated by elk PrPcwd conversion of YFP-PrPc on the B-cell line to YFP-PrPcwd, a stably transfected cell line will be developed and validated as a sensitive, specific, and reproducible bioassay for detecting PrPcwd infected elk and deer tissues and body fluids during Phase II.

* information listed above is at the time of submission.

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