Ruminant B-Lymphocyte Yellow Fluorescent Protein Aggregation Bioassay for Elk Chronic Wasting Disease

Award Information
Agency: Department of Defense
Branch: Army
Contract: W81XWH-04-C-0141
Agency Tracking Number: A045-027-0237
Amount: $100,000.00
Phase: Phase I
Program: STTR
Awards Year: 2004
Solicitation Year: 2004
Solicitation Topic Code: A04-T027
Solicitation Number: N/A
Small Business Information
1024 S. Innovation Way, Stillwater, OK, 74074
DUNS: 879735579
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 David Goad
 Principal Investigator
 (405) 372-9535
 dgoad@nomadics.com
Business Contact
 James Luby
Title: Chief Operating Officer
Phone: (405) 372-9535
Email: jluby@nomadics.com
Research Institution
 Oklahoma State University
 Kenneth Clinkenbeard
 College of Veterinary Medicine, 221 McElroy Hall
Stillwater, OK, 74078
 (405) 744-4467
 Nonprofit college or university
Abstract
The goal of the proposed research is to develop a cell culture model for elk chronic wasting disease (CWD) prion propagation that can be used as a bioassay for detecting CWD. Our goal will be accomplished by bioengineering a bovine B-lymphocyte cell (B-cell) line to surface express elk PrPc fused to yellow fluorescent protein (YFP). Interaction of these B-cells with infectious, mis-folded, protease resistant CWD prion (PrPcwd) will induce conversion of the dispersed YFP-PrPc to aggregated mis-folded YFP-PrPcwd that will be detected by confocal microscopy as aggregated YFP-PrPcwd on the B-cell surface. Once Phase I proof-of-concept has been demonstrated by elk PrPcwd conversion of YFP-PrPc on the B-cell line to YFP-PrPcwd, a stably transfected cell line will be developed and validated as a sensitive, specific, and reproducible bioassay for detecting PrPcwd infected elk and deer tissues and body fluids during Phase II.

* Information listed above is at the time of submission. *

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