Fluorescence-based Multiplexed Universal Virus Detection Platform

Award Information
Agency: Department of Defense
Branch: Army
Contract: W81XWH-06-C-0048
Agency Tracking Number: A052-165-0477
Amount: $69,999.00
Phase: Phase I
Program: SBIR
Awards Year: 2005
Solicitation Year: 2005
Solicitation Topic Code: A05-165
Solicitation Number: 2005.2
Small Business Information
1024 S. Innovation Way, Stillwater, OK, 74074
DUNS: 188613918
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Akhilesh Ramachandran
 Principal Investigator
 (405) 372-9535
 aramachandran@nomadics.com
Business Contact
 Jim Luby
Title: COO
Phone: (405) 372-9535
Email: jluby@nomadics.com
Research Institution
N/A
Abstract
The aim of this study is to develop a multiplexed virus detection platform capable of detecting within three hours the presence of any known virus, genetically modified virus or unidentified virus, in clinical samples like serum. A PCR based technique will be employed to identify the presence of unique viral sequences. In its ultimate form, the proposed system will encompass a simple and rapid sample preparation step followed by PCR, fluorophore tagging (using a highly stable and novel amplifying fluorescent polymer), and hybridization to a virus-specific oligonucleotide microarray printed on the cap of a PCR tube. The system will also allow for the cloning and subsequent sequencing of nucleic acids to establish the presence of unknown viruses. This diagnostic platform will find applications in screening banked blood for the presence of viruses, detecting the presence of unnatural (mutated) viruses used as bio-warfare agents, and detecting the presence of latent viruses in the human system. In Phase 1 we will perform the following objectives: 1) Standardize and optimize the PCR, 2) Test and optimize the sample preparation protocol, 3) Fabricate microarrays, 4) Optimize microarray hybridization, 5) Clone and sequence PCR products, and 6) Evaluate the sensitivity and specificity of the assay.

* Information listed above is at the time of submission. *

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