Antibodies Engineered for Allosteric Control of Binding.

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 1R43AI049006-01A1
Amount: $100,000.00
Phase: Phase I
Program: SBIR
Awards Year: 2001
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
1682 CALICO CIR, POCATELLO, ID, 83201
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 IAIN PETERS
 () -
Business Contact
Phone: (208) 237-2541
Email: PETERLOUI@ISU.EDU
Research Institution
N/A
Abstract
DESCRIPTION (provided by applicant): Most current purification methodologies are very expensive due to the requirement for multiple steps and empirical development. In principle, immuno-affinity chromatography (IAC) should provide a very attractive alternative as it affords remarkable enrichment in a single step and is easily adapted to any target compound. Unfortunately however, IAC has seen rather restricted use due to difficulty eluting captured ligand without inactivating the antibody and/or product. Generally a large number of candidate antibodies must be screened to find one that releases the antigen under sufficiently mild conditions. Even then, elution usually causes some antibody inactivation, making IAC operationally expensive due to the need for periodic column replacement. The proposed research will test efficacy of a molecular design capable of imposing allosteric regulation on any scFv. Elution conditions of IAC utilizing this technology are truly mild as allosteric regulator binding causes the scFv to adopt a low activity conformation that is readily accessible through flexibility inherent in its specialized structure. Consequently, no scFv unfolding occurs and full activity should be retained over numerous chromatography cycles. Furthermore, efficacy of the design is expected to facilitate utilization of even very high affinity scFv (eg. 10-11 M) in IAC. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

* Information listed above is at the time of submission. *

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