AND D-MYOINOSITOL-1,4,5-TRIPHOSPHATE AND THEIR ANALOGS THE LONG-TERM OBJECTIVE OF THIS PROPOSAL ARE: (1) TO DEVELOP A GENERAL METHOD FOR THE SYNTHESIS OF PHOSPHATIDYL- D-MYOINOSITOL-4,5-BISPHOSPHATE [PTDINS(4,5)P2] AS A KEY MATERIAL FOR THE PREPARATION

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1986
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
4714
Agency Tracking Number:
4714
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Cornell Univ Research Park, 270-276 Langmuir Laboratory, Ithaca, NY, 14850
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
DR R ANEJA
PRINCIPAL INVESTIGATOR
(607) 257-1166
Business Contact:
() -
Research Institution:
n/a
Abstract
AND D-MYOINOSITOL-1,4,5-TRIPHOSPHATE AND THEIR ANALOGS THE LONG-TERM OBJECTIVE OF THIS PROPOSAL ARE: (1) TO DEVELOP A GENERAL METHOD FOR THE SYNTHESIS OF PHOSPHATIDYL- D-MYOINOSITOL-4,5-BISPHOSPHATE [PTDINS(4,5)P2] AS A KEY MATERIAL FOR THE PREPARATION OF D-MYO-INOSITOL-1,4,5- TRIPHOSPHATE [INS(1,4,5)P2] AND A VARIETY OF ANALOGS; (2) TODESIGN AND PREPARE THESE COMPOUNDS; AND (3) TO EVALUATE THEIR APPLICATIONS FOR INVESTIGATING THE MECHANISM OF HORMONE-INDUCED INTRACELLULAR CA2+ RELEASE AND SIGNAL TRANSMISSION. A GENERAL SCHEME OF SYNTHESIS STARTING FROM NATURAL PHOSPHATIDYLINOSITOL [PTDINS] IS PROPOSED. DURING PHASE I, USING A RELAY STRATEGY, PTDINS(4,5)P2 FROM BEEF- BRAIN PHOSPHOINOSITIDES WILL BE CONVERTED INTO 1-AMINODEOXY-GLYCEROPHOSPHO-D-MYO-INOSITOL-4',5'-BISPHOSPHATE, AND GLYCEROPHOSPHOINOSITOL-4,5-BISPHOSPHATE. THE TWO COMPOUNDS WILL THEN BE COMPARED IN MODEL STUDIES AS CANDIDATE ANALOGS FOR LINKING TO PHOTOLABILE GROUPS, AND TO INERT SUPPORTS FORUSE AS POTENTIAL AIDS FOR THE CHARACTERIZATION AND PURIFICATION OF THE INS(1,4,5)P3-BINDING PROTEIN. ALSO DURING PHASE I, KEY STEPS IN THE PROPOSED GENERAL SCHEME WILL BE INVESTIGATED TO EXPERIMENTALLY DETERMINE THE FEASIBILITY OF THE OVERALL STRATEGY FOR SYNTHESIS STARTING FROM PTDINS.

* information listed above is at the time of submission.

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