PRACTICAL SYNTHESIS OF PHOSPHOINOSITIDES

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$500,000.00
Award Year:
1989
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
4945
Agency Tracking Number:
4945
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
270-276 Langmuir Laboratory, Brown Road, Ithaca, NY, 14850
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Dr Rajindra Aneja
Principal Investigator
(607) 257-1166
Business Contact:
() -
Research Institute:
n/a
Abstract
THERE IS A CURRENT AND GROWING INTEREST IN THE ROLE OF MEMBRANE PHOSPHOINOSITIDES IN CELL BIOLOGY, ESPECIALLY IN RELATION TO THE TRANSMISSION OF CA2+ MEDIATED SIGNALS. THESE SIGNALS ARE GENERATED WHEN CELL-SURFACE RECEPTORS ARE ACTIVATED BY HORMONES, NEUROTRANSMITTERS, GROWTH FACTORS, AND OTHER AGONISTS, IN RELATION TO THE MECHANISM OF MALIGNANT TRANSFORMATIONS INDUCED BY ONCOGENES AND BY THE ACTIVATION OF KINASE C. SAMPLES OF PURE PHOSPHOINOSITIDES AND ANALOGS ARE IMPORTANT FOR RESEARCH AND AS POTENTIAL THERAPEUTIC AGENTS. PRACTICAL METHODS FOR THEIR PREPARATIONBY SYNTHESIS ARE NOT AVAILABLE, HOWEVER. THE OVERALL OBJECTIVE OF THIS PROPOSAL IS TO DEVELOP A GENERAL, SIMPLE, AND PRACTICAL METHOD FOR THE SYNTHESIS OF DISTINCT MOLECULARSPECIES OF PHOSPHATIDYLINOSITOL (PI), PHOSPHATIDYLINOSITOL- 4-PHOSPHATE (PIP), AND PHOSPHATIDYLINOSITOL-4,5- BISPHOSPHATE (PIP2), EACH CARRYING FATTYACYL RESIDUES OF CHOICE IN POSITIONS 1',2'. THE MIXTURES OF FATTYACYLS IN NATURAL PI AND DERIVATIVES WITH ANY DESIRED FATTYACYLS WILL BE REPLACED BY A DEACYLATION-REACYLATION CYCLE, OR BY REMOVAL AND REINTRODUCTION OF THE COMPLETE DIGLYCERIDE RESIDUE. THE SYNTHESIS DEPENDS ON AN INNOVATIVE APPLICATIONOF LIPASES IN A COMPOSITE CHEMICAL AND ENZYMATIC SEQUENCE. DURING PHASE I, THIS ASPECT AND THE PREREQUISITE PROTECTION AND DEBLOCKING OF THE INOSITOL HYDROXYLS WILL BE EVALUATED FOR THE PREPARATION OF SEMISYNTHETIC 1',2'-DIACYL-SN- GLYCEROPHOSPHOINOSITOLS FROM PI ISOLATED FROM SOY-LECITHIN, TO DETERMINE THE FEASIBILITY OF THE GENERAL STRATEGY.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government