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Soft Cleaning of Wire-Immobilized Genetic Material

Award Information
Agency: National Aeronautics and Space Administration
Branch: N/A
Contract: 80NSSC23PB508
Agency Tracking Number: 232121
Amount: $156,421.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: H3
Solicitation Number: SBIR_23_P1
Solicitation Year: 2023
Award Year: 2023
Award Start Date (Proposal Award Date): 2023-07-27
Award End Date (Contract End Date): 2024-02-02
Small Business Information
7047 Madison Pike, Suite 305
Huntsville, AL 35806-2197
United States
DUNS: 968887195
HUBZone Owned: No
Woman Owned: Yes
Socially and Economically Disadvantaged: No
Principal Investigator
 Niel Crews
 (318) 243-6942
Business Contact
 Heather Johns
Phone: (256) 327-9012
Research Institution

To automate the sample prep for MinION sequencing, we propose a paradigm shift in the inherently liquid-based, multi-step protocol. The traditionally labor-intense process will occur within disposable microfluidic ldquo;cardsrdquo; which have an unexpected feature. Each card will contain separate reaction wells where single operations will occur (e.g. lysis, fragmentation, amplification). However, these chambers will all remain physically isolated from one another, having no active fluidic interconnects between them. The target DNA will undergo each successive modification step ndash; moving from reaction well to reaction well ndash; simply by going through the intervening walls. This technique, which we call Soft Cleaning of Wire-Immobilized Genetic material (SCWI-G), constitutes a mechanical method of isolating and purifying nucleic acids.

* Information listed above is at the time of submission. *

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